Label-free, in situ SERS monitoring of individual DNA hybridization in microfluidics

Ji Qi; Jianbo Zeng; Fusheng Zhao; Steven Hsesheng Lin; Balakrishnan Raja; Ulrich Strych; Richard C. Willson; Wei Chuan Shih

doi:10.1039/c4nr01951b

Label-free, in situ SERS monitoring of individual DNA hybridization in microfluidics

Ji Qi, Jianbo Zeng, Fusheng Zhao, Steven Hsesheng Lin, Balakrishnan Raja, Ulrich Strych, Richard C. Willson, Wei Chuan Shih

Houston Methodist

Research output: Contribution to journal › Article › peer-review

85 Scopus citations

Abstract

We present label-free, in situ monitoring of individual DNA hybridization in microfluidics. By immobilizing molecular sentinel probes on nanoporous gold disks, we demonstrate sensitivity approaching the single-molecule limit via surface-enhanced Raman scattering which provides robust signals without photobleaching for more than an hour. We further demonstrate that a target concentration as low as 20 pM can be detected within 10 min under diffusion-limited transport. This journal is

Original language	English (US)
Pages (from-to)	8521-8526
Number of pages	6
Journal	Nanoscale
Volume	6
Issue number	15
DOIs	https://doi.org/10.1039/c4nr01951b
State	Published - Aug 7 2014

ASJC Scopus subject areas

General Materials Science

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10.1039/c4nr01951b

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abstract = "We present label-free, in situ monitoring of individual DNA hybridization in microfluidics. By immobilizing molecular sentinel probes on nanoporous gold disks, we demonstrate sensitivity approaching the single-molecule limit via surface-enhanced Raman scattering which provides robust signals without photobleaching for more than an hour. We further demonstrate that a target concentration as low as 20 pM can be detected within 10 min under diffusion-limited transport. This journal is",

author = "Ji Qi and Jianbo Zeng and Fusheng Zhao and Lin, \{Steven Hsesheng\} and Balakrishnan Raja and Ulrich Strych and Willson, \{Richard C.\} and Shih, \{Wei Chuan\}",

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doi = "10.1039/c4nr01951b",

language = "English (US)",

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AU - Qi, Ji

AU - Zeng, Jianbo

AU - Zhao, Fusheng

AU - Lin, Steven Hsesheng

AU - Raja, Balakrishnan

AU - Strych, Ulrich

AU - Willson, Richard C.

AU - Shih, Wei Chuan

PY - 2014/8/7

Y1 - 2014/8/7

N2 - We present label-free, in situ monitoring of individual DNA hybridization in microfluidics. By immobilizing molecular sentinel probes on nanoporous gold disks, we demonstrate sensitivity approaching the single-molecule limit via surface-enhanced Raman scattering which provides robust signals without photobleaching for more than an hour. We further demonstrate that a target concentration as low as 20 pM can be detected within 10 min under diffusion-limited transport. This journal is

AB - We present label-free, in situ monitoring of individual DNA hybridization in microfluidics. By immobilizing molecular sentinel probes on nanoporous gold disks, we demonstrate sensitivity approaching the single-molecule limit via surface-enhanced Raman scattering which provides robust signals without photobleaching for more than an hour. We further demonstrate that a target concentration as low as 20 pM can be detected within 10 min under diffusion-limited transport. This journal is

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JO - Nanoscale

JF - Nanoscale

IS - 15

ER -

Label-free, in situ SERS monitoring of individual DNA hybridization in microfluidics

Abstract

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