Label-free, in situ SERS monitoring of individual DNA hybridization in microfluidics

Ji Qi; Jianbo Zeng; Fusheng Zhao; Steven Hsesheng Lin; Balakrishnan Raja; Ulrich Strych; Richard C. Willson; Wei Chuan Shih

doi:10.1039/c4nr01951b

Label-free, in situ SERS monitoring of individual DNA hybridization in microfluidics

Ji Qi, Jianbo Zeng, Fusheng Zhao, Steven Hsesheng Lin, Balakrishnan Raja, Ulrich Strych, Richard C. Willson, Wei Chuan Shih

Research output: Contribution to journal › Article › peer-review

79 Scopus citations

Abstract

We present label-free, in situ monitoring of individual DNA hybridization in microfluidics. By immobilizing molecular sentinel probes on nanoporous gold disks, we demonstrate sensitivity approaching the single-molecule limit via surface-enhanced Raman scattering which provides robust signals without photobleaching for more than an hour. We further demonstrate that a target concentration as low as 20 pM can be detected within 10 min under diffusion-limited transport. This journal is

Original language	English (US)
Pages (from-to)	8521-8526
Number of pages	6
Journal	Nanoscale
Volume	6
Issue number	15
DOIs	https://doi.org/10.1039/c4nr01951b
State	Published - Aug 7 2014

ASJC Scopus subject areas

Materials Science(all)

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10.1039/c4nr01951b

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abstract = "We present label-free, in situ monitoring of individual DNA hybridization in microfluidics. By immobilizing molecular sentinel probes on nanoporous gold disks, we demonstrate sensitivity approaching the single-molecule limit via surface-enhanced Raman scattering which provides robust signals without photobleaching for more than an hour. We further demonstrate that a target concentration as low as 20 pM can be detected within 10 min under diffusion-limited transport. This journal is",

author = "Ji Qi and Jianbo Zeng and Fusheng Zhao and Lin, {Steven Hsesheng} and Balakrishnan Raja and Ulrich Strych and Willson, {Richard C.} and Shih, {Wei Chuan}",

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doi = "10.1039/c4nr01951b",

language = "English (US)",

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AU - Qi, Ji

AU - Zeng, Jianbo

AU - Zhao, Fusheng

AU - Lin, Steven Hsesheng

AU - Raja, Balakrishnan

AU - Strych, Ulrich

AU - Willson, Richard C.

AU - Shih, Wei Chuan

PY - 2014/8/7

Y1 - 2014/8/7

N2 - We present label-free, in situ monitoring of individual DNA hybridization in microfluidics. By immobilizing molecular sentinel probes on nanoporous gold disks, we demonstrate sensitivity approaching the single-molecule limit via surface-enhanced Raman scattering which provides robust signals without photobleaching for more than an hour. We further demonstrate that a target concentration as low as 20 pM can be detected within 10 min under diffusion-limited transport. This journal is

AB - We present label-free, in situ monitoring of individual DNA hybridization in microfluidics. By immobilizing molecular sentinel probes on nanoporous gold disks, we demonstrate sensitivity approaching the single-molecule limit via surface-enhanced Raman scattering which provides robust signals without photobleaching for more than an hour. We further demonstrate that a target concentration as low as 20 pM can be detected within 10 min under diffusion-limited transport. This journal is

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Label-free, in situ SERS monitoring of individual DNA hybridization in microfluidics

Abstract

ASJC Scopus subject areas

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