Label-free, in situ SERS monitoring of individual DNA hybridization in microfluidics

Ji Qi, Jianbo Zeng, Fusheng Zhao, Steven Hsesheng Lin, Balakrishnan Raja, Ulrich Strych, Richard C. Willson, Wei Chuan Shih

Research output: Contribution to journalArticlepeer-review

75 Scopus citations

Abstract

We present label-free, in situ monitoring of individual DNA hybridization in microfluidics. By immobilizing molecular sentinel probes on nanoporous gold disks, we demonstrate sensitivity approaching the single-molecule limit via surface-enhanced Raman scattering which provides robust signals without photobleaching for more than an hour. We further demonstrate that a target concentration as low as 20 pM can be detected within 10 min under diffusion-limited transport. This journal is

Original languageEnglish (US)
Pages (from-to)8521-8526
Number of pages6
JournalNanoscale
Volume6
Issue number15
DOIs
StatePublished - Aug 7 2014

ASJC Scopus subject areas

  • Materials Science(all)

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