Label-free detection of Staphylococcus aureus captured on immutable ligand arrays

Avijit K. Adak, J. William Boley, David P. Lyvers, George T. Chiu, Philip Low, Ronald Reifenberger, Alexander Wei

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

The rapid capture and label-free detection of Staphylococcus aureus, an opportunistic bacterium that can infect upon contact, can be performed using periodic microarrays of ligand-protein conjugates created by noncontact (inkjet) printing, darkfield imaging conditions, and a FFT-based readout method. Ink solutes were prepared using bovine serum albumin (BSA) conjugated to a glycan with high affinity for bacterial adhesins and printed as dot-matrix arrays with periodicities of 80-120 μm using a thermal injection method. Upon exposing the glycan-BSA microarrays to live strains of S. aureus, patterns emerge that can be detected under optical darkfield conditions. These patterns can be decoded by fast Fourier transform (FFT) analysis to generate fault-tolerant readout signals that correspond to the capture of S. aureus, with a limit of detection between 102 and 103 cfu/mL. Inkjet printing provides independent control over array periodicity, enabling FFT signals to be assigned to specific frequencies in reciprocal k-space.

Original languageEnglish (US)
Pages (from-to)6404-6411
Number of pages8
JournalACS Applied Materials and Interfaces
Volume5
Issue number13
DOIs
StatePublished - Jul 10 2013

Keywords

  • biosensors
  • Fourier transform
  • glycans
  • inkjet printing
  • microarrays
  • pathogens

ASJC Scopus subject areas

  • Materials Science(all)
  • Medicine(all)

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