Label-free and self-assembled fluorescent DNA nanopompom for determination of miRNA-21

Nandi Chen; Junyu Li; Xianzhen Feng; Yanping Yang; Li Zhu; Xiaomeng Chen; Xuan Liu; Yang Li; Cunchuan Wang; Ligang Xia

doi:10.1007/s00604-020-04377-6

Label-free and self-assembled fluorescent DNA nanopompom for determination of miRNA-21

Nandi Chen, Junyu Li, Xianzhen Feng, Yanping Yang, Li Zhu, Xiaomeng Chen, Xuan Liu, Yang Li, Cunchuan Wang, Ligang Xia

Houston Methodist

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

A label-free fluorescence method based on self-assembled DNA nanopompom has been developed for miRNA-21 detection. In the presence of miRNA-21, three DNA hairpin probes with split G-quadruplex assemble the DNA nanopompom. Based on the isothermal toehold-mediated DNA strand displacement reaction, the target miRNA can be catalytically recycled and trigger three DNA hairpin probes to self-assemble the DNA nanopompom and release the G-quadruplex. The formation of the G-quadruplex increases the fluorescence emission intensity of thioflavin. For thioflavin-based miRNA-21 detection, the excitation and emission wavelengths are set to 425 nm and 490 nm, respectively. The limit of detection for miRNA-21 is 0.8 pM according to F/F0 = 0.0031 × C_miRNA-21 + 1.0382 (R² = 0.9978). This sensing system provides a low-cost, effective, and convenient method for miRNA detection, which holds great potential in biochemical diagnosis and clinical practice. [Figure not available: see fulltext.].

Original language	English (US)
Article number	432
Journal	Microchimica Acta
Volume	187
Issue number	8
DOIs	https://doi.org/10.1007/s00604-020-04377-6
State	Published - Aug 1 2020

Keywords

DNA nanotechnology
G-quadruplex
Label-free detection
miRNA
Self-assemble

ASJC Scopus subject areas

Analytical Chemistry

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10.1007/s00604-020-04377-6

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@article{0ef3c881b9074b04ab6c0826d98d124d,

title = "Label-free and self-assembled fluorescent DNA nanopompom for determination of miRNA-21",

abstract = "A label-free fluorescence method based on self-assembled DNA nanopompom has been developed for miRNA-21 detection. In the presence of miRNA-21, three DNA hairpin probes with split G-quadruplex assemble the DNA nanopompom. Based on the isothermal toehold-mediated DNA strand displacement reaction, the target miRNA can be catalytically recycled and trigger three DNA hairpin probes to self-assemble the DNA nanopompom and release the G-quadruplex. The formation of the G-quadruplex increases the fluorescence emission intensity of thioflavin. For thioflavin-based miRNA-21 detection, the excitation and emission wavelengths are set to 425 nm and 490 nm, respectively. The limit of detection for miRNA-21 is 0.8 pM according to F/F0 = 0.0031 × CmiRNA-21 + 1.0382 (R2 = 0.9978). This sensing system provides a low-cost, effective, and convenient method for miRNA detection, which holds great potential in biochemical diagnosis and clinical practice. [Figure not available: see fulltext.].",

keywords = "DNA nanotechnology, G-quadruplex, Label-free detection, miRNA, Self-assemble",

author = "Nandi Chen and Junyu Li and Xianzhen Feng and Yanping Yang and Li Zhu and Xiaomeng Chen and Xuan Liu and Yang Li and Cunchuan Wang and Ligang Xia",

note = "Funding Information: This research was supported by the National Natural Science Foundation of China (Grant No. 81971637), the Guangdong Basic and Applied Basic Research Foundation (2019A1515110402), and the Technology \& Innovation Commission of Shenzhen Municipality (Shenzhen, China; Grant No. JCYJ20190807145011340). Funding Information: This research was supported by the National Natural Science Foundation of China (Grant No. 81971637), the Guangdong Basic and Applied Basic Research Foundation (2019A1515110402), and the Technology \& Innovation Commission of Shenzhen Municipality (Shenzhen, China; Grant No. JCYJ20190807145011340). Publisher Copyright: {\textcopyright} 2020, Springer-Verlag GmbH Austria, part of Springer Nature.",

year = "2020",

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doi = "10.1007/s00604-020-04377-6",

language = "English (US)",

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journal = "Microchimica Acta",

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T1 - Label-free and self-assembled fluorescent DNA nanopompom for determination of miRNA-21

AU - Chen, Nandi

AU - Li, Junyu

AU - Feng, Xianzhen

AU - Yang, Yanping

AU - Zhu, Li

AU - Chen, Xiaomeng

AU - Liu, Xuan

AU - Li, Yang

AU - Wang, Cunchuan

AU - Xia, Ligang

N1 - Funding Information: This research was supported by the National Natural Science Foundation of China (Grant No. 81971637), the Guangdong Basic and Applied Basic Research Foundation (2019A1515110402), and the Technology & Innovation Commission of Shenzhen Municipality (Shenzhen, China; Grant No. JCYJ20190807145011340). Funding Information: This research was supported by the National Natural Science Foundation of China (Grant No. 81971637), the Guangdong Basic and Applied Basic Research Foundation (2019A1515110402), and the Technology & Innovation Commission of Shenzhen Municipality (Shenzhen, China; Grant No. JCYJ20190807145011340). Publisher Copyright: © 2020, Springer-Verlag GmbH Austria, part of Springer Nature.

PY - 2020/8/1

Y1 - 2020/8/1

N2 - A label-free fluorescence method based on self-assembled DNA nanopompom has been developed for miRNA-21 detection. In the presence of miRNA-21, three DNA hairpin probes with split G-quadruplex assemble the DNA nanopompom. Based on the isothermal toehold-mediated DNA strand displacement reaction, the target miRNA can be catalytically recycled and trigger three DNA hairpin probes to self-assemble the DNA nanopompom and release the G-quadruplex. The formation of the G-quadruplex increases the fluorescence emission intensity of thioflavin. For thioflavin-based miRNA-21 detection, the excitation and emission wavelengths are set to 425 nm and 490 nm, respectively. The limit of detection for miRNA-21 is 0.8 pM according to F/F0 = 0.0031 × CmiRNA-21 + 1.0382 (R2 = 0.9978). This sensing system provides a low-cost, effective, and convenient method for miRNA detection, which holds great potential in biochemical diagnosis and clinical practice. [Figure not available: see fulltext.].

AB - A label-free fluorescence method based on self-assembled DNA nanopompom has been developed for miRNA-21 detection. In the presence of miRNA-21, three DNA hairpin probes with split G-quadruplex assemble the DNA nanopompom. Based on the isothermal toehold-mediated DNA strand displacement reaction, the target miRNA can be catalytically recycled and trigger three DNA hairpin probes to self-assemble the DNA nanopompom and release the G-quadruplex. The formation of the G-quadruplex increases the fluorescence emission intensity of thioflavin. For thioflavin-based miRNA-21 detection, the excitation and emission wavelengths are set to 425 nm and 490 nm, respectively. The limit of detection for miRNA-21 is 0.8 pM according to F/F0 = 0.0031 × CmiRNA-21 + 1.0382 (R2 = 0.9978). This sensing system provides a low-cost, effective, and convenient method for miRNA detection, which holds great potential in biochemical diagnosis and clinical practice. [Figure not available: see fulltext.].

KW - DNA nanotechnology

KW - G-quadruplex

KW - Label-free detection

KW - miRNA

KW - Self-assemble

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SN - 0026-3672

VL - 187

JO - Microchimica Acta

JF - Microchimica Acta

IS - 8

M1 - 432

ER -

Label-free and self-assembled fluorescent DNA nanopompom for determination of miRNA-21

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Keywords

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