Kinetics of spontaneous and plasma-stimulated sphingomyelin transfer

Henry J. Pownall; Diane Hickson; Antonio Gotto; John B. Massey

doi:10.1016/0005-2760(82)90099-6

Kinetics of spontaneous and plasma-stimulated sphingomyelin transfer

Henry J. Pownall, Diane Hickson, Antonio Gotto, John B. Massey

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

The mechanism of transfer of a pyrene-labeled sphingomyelin (PySM) between different lipid compartments was studied by a fluorescence technique. The first-order kinetics are independent of donor and acceptor concentration and the identity of the acceptor; the rates are accelerated by 'structure-breaking' solutes and inhibited by 'structure-making' solutes. These observations are consistent with the transfer of PySM occurring via the aqueous phase that separates the donor and acceptor compartments. We have partially purified a plasma factor that stimulates the transfer rate. Our in vitro results suggest that both spontaneous and stimulated transfer might contribute to the redistribution of sphingomyelin in vivo.

Original language	English (US)
Pages (from-to)	169-176
Number of pages	8
Journal	Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume	712
Issue number	1
DOIs	https://doi.org/10.1016/0005-2760(82)90099-6
State	Published - Jul 20 1982

Keywords

Lipid transfer
Plasma factor kinetics
Sphingomyelin

ASJC Scopus subject areas

Endocrinology
Biophysics
Biochemistry
General Medicine

Divisions

Endocrinology, Diabetes and Metabolism

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10.1016/0005-2760(82)90099-6

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title = "Kinetics of spontaneous and plasma-stimulated sphingomyelin transfer",

abstract = "The mechanism of transfer of a pyrene-labeled sphingomyelin (PySM) between different lipid compartments was studied by a fluorescence technique. The first-order kinetics are independent of donor and acceptor concentration and the identity of the acceptor; the rates are accelerated by 'structure-breaking' solutes and inhibited by 'structure-making' solutes. These observations are consistent with the transfer of PySM occurring via the aqueous phase that separates the donor and acceptor compartments. We have partially purified a plasma factor that stimulates the transfer rate. Our in vitro results suggest that both spontaneous and stimulated transfer might contribute to the redistribution of sphingomyelin in vivo.",

keywords = "Lipid transfer, Plasma factor kinetics, Sphingomyelin",

author = "Pownall, \{Henry J.\} and Diane Hickson and Antonio Gotto and Massey, \{John B.\}",

note = "Funding Information: The authors wish to thank Ms. Sarah Myers for assistance in the preparation of the manuscript and Ms. Susan McNeely for providing the line drawings. We also thank Dr. Louis C. Smith for his enthusiastic support and critical reading of the manuscript. This research was supported by a grant from the National Institutes of Health (HL-26250) and was developed by the Atherosclerosis, Lipids and Lipoprotein Section of the National Heart and Blood Vessel Research and Demonstration Center (HL-17269) a grant-supported research project of the National Institutes of Health. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.",

year = "1982",

month = jul,

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doi = "10.1016/0005-2760(82)90099-6",

language = "English (US)",

volume = "712",

pages = "169--176",

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TY - JOUR

T1 - Kinetics of spontaneous and plasma-stimulated sphingomyelin transfer

AU - Pownall, Henry J.

AU - Hickson, Diane

AU - Gotto, Antonio

AU - Massey, John B.

N1 - Funding Information: The authors wish to thank Ms. Sarah Myers for assistance in the preparation of the manuscript and Ms. Susan McNeely for providing the line drawings. We also thank Dr. Louis C. Smith for his enthusiastic support and critical reading of the manuscript. This research was supported by a grant from the National Institutes of Health (HL-26250) and was developed by the Atherosclerosis, Lipids and Lipoprotein Section of the National Heart and Blood Vessel Research and Demonstration Center (HL-17269) a grant-supported research project of the National Institutes of Health. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.

PY - 1982/7/20

Y1 - 1982/7/20

N2 - The mechanism of transfer of a pyrene-labeled sphingomyelin (PySM) between different lipid compartments was studied by a fluorescence technique. The first-order kinetics are independent of donor and acceptor concentration and the identity of the acceptor; the rates are accelerated by 'structure-breaking' solutes and inhibited by 'structure-making' solutes. These observations are consistent with the transfer of PySM occurring via the aqueous phase that separates the donor and acceptor compartments. We have partially purified a plasma factor that stimulates the transfer rate. Our in vitro results suggest that both spontaneous and stimulated transfer might contribute to the redistribution of sphingomyelin in vivo.

AB - The mechanism of transfer of a pyrene-labeled sphingomyelin (PySM) between different lipid compartments was studied by a fluorescence technique. The first-order kinetics are independent of donor and acceptor concentration and the identity of the acceptor; the rates are accelerated by 'structure-breaking' solutes and inhibited by 'structure-making' solutes. These observations are consistent with the transfer of PySM occurring via the aqueous phase that separates the donor and acceptor compartments. We have partially purified a plasma factor that stimulates the transfer rate. Our in vitro results suggest that both spontaneous and stimulated transfer might contribute to the redistribution of sphingomyelin in vivo.

KW - Lipid transfer

KW - Plasma factor kinetics

KW - Sphingomyelin

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DO - 10.1016/0005-2760(82)90099-6

M3 - Article

C2 - 6810944

AN - SCOPUS:0019998166

SN - 0005-2760

VL - 712

SP - 169

EP - 176

JO - Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism

JF - Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism

IS - 1

ER -

Kinetics of spontaneous and plasma-stimulated sphingomyelin transfer

Abstract

Keywords

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