Abstract
The mechanism of transfer of a pyrene-labeled sphingomyelin (PySM) between different lipid compartments was studied by a fluorescence technique. The first-order kinetics are independent of donor and acceptor concentration and the identity of the acceptor; the rates are accelerated by 'structure-breaking' solutes and inhibited by 'structure-making' solutes. These observations are consistent with the transfer of PySM occurring via the aqueous phase that separates the donor and acceptor compartments. We have partially purified a plasma factor that stimulates the transfer rate. Our in vitro results suggest that both spontaneous and stimulated transfer might contribute to the redistribution of sphingomyelin in vivo.
Original language | English (US) |
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Pages (from-to) | 169-176 |
Number of pages | 8 |
Journal | Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism |
Volume | 712 |
Issue number | 1 |
DOIs | |
State | Published - Jul 20 1982 |
Keywords
- Lipid transfer
- Plasma factor kinetics
- Sphingomyelin
ASJC Scopus subject areas
- Endocrinology
- Biophysics
- Biochemistry
- Medicine(all)