Kinetics of spontaneous and plasma-stimulated sphingomyelin transfer

Henry J. Pownall, Diane Hickson, Antonio Gotto, John B. Massey

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

The mechanism of transfer of a pyrene-labeled sphingomyelin (PySM) between different lipid compartments was studied by a fluorescence technique. The first-order kinetics are independent of donor and acceptor concentration and the identity of the acceptor; the rates are accelerated by 'structure-breaking' solutes and inhibited by 'structure-making' solutes. These observations are consistent with the transfer of PySM occurring via the aqueous phase that separates the donor and acceptor compartments. We have partially purified a plasma factor that stimulates the transfer rate. Our in vitro results suggest that both spontaneous and stimulated transfer might contribute to the redistribution of sphingomyelin in vivo.

Original languageEnglish (US)
Pages (from-to)169-176
Number of pages8
JournalBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume712
Issue number1
DOIs
StatePublished - Jul 20 1982

Keywords

  • Lipid transfer
  • Plasma factor kinetics
  • Sphingomyelin

ASJC Scopus subject areas

  • Endocrinology
  • Biophysics
  • Biochemistry
  • Medicine(all)

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