TY - GEN
T1 - KINETIC ENHANCEMENT OF RECEPTOR-LIGAND INTERACTIONS IN MODULAR GLASS MICROFLUIDIC BIOASSAY DEVICES
AU - Sathish, Shivani
AU - Shen, Amy Q.
N1 - Funding Information:
S.S. is a JSPS DC2 fellow and this work is supported by JSPS KAKENHI [Grant Number 19J11009]. We thank Mr. Kazumi Toda-Peters for the technical assistance with device fabrication.
Publisher Copyright:
© 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
PY - 2021
Y1 - 2021
N2 - Microfluidic bioassay systems rely on the quantitative detection of ligands flowing in a liquid sample, by exploiting their innate nature to react with specific receptors immobilized on the microchannel walls. In these systems, the effective ligand detection time is either limited by the receptor affinity or by the rate of ligand replenishment close to the receptor-coated surface. In this study, we utilize a novel 3D modular microfluidic confinement device that enables the use of extremely fast flows to overcome mass transport limitations, thereby allowing rapid (< 10 mins) and sensitive (limit of detection: ~10 pM) ligand detection.
AB - Microfluidic bioassay systems rely on the quantitative detection of ligands flowing in a liquid sample, by exploiting their innate nature to react with specific receptors immobilized on the microchannel walls. In these systems, the effective ligand detection time is either limited by the receptor affinity or by the rate of ligand replenishment close to the receptor-coated surface. In this study, we utilize a novel 3D modular microfluidic confinement device that enables the use of extremely fast flows to overcome mass transport limitations, thereby allowing rapid (< 10 mins) and sensitive (limit of detection: ~10 pM) ligand detection.
KW - kinetic enhancement
KW - modular microfluidic device
KW - rapid ligand detection
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M3 - Conference contribution
AN - SCOPUS:85136956952
T3 - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 1119
EP - 1120
BT - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Y2 - 10 October 2021 through 14 October 2021
ER -