TY - JOUR
T1 - Isolation of neural stem cells from neural tissues using the neurosphere technique
AU - Ferrari, Daniela
AU - Binda, Elena
AU - De Filippis, Lidia
AU - Vescovi, Angelo
PY - 2010/1/1
Y1 - 2010/1/1
N2 - This unit describes protocols for the derivation, characterization, and expansion of neural stem cell (NSC) lines from the adult mouse subvetricular zone (mNSCs), embryonic mouse brain and from the human fetal brain (hNSCs). NSCs can be isolated by enzymatic digestion of specific regions (NSCs niches) of the central nervous system (CNS) and grown in suspension. By using this methodology, NSCs form spherical clusters called neuropsheres, which are mechanically dissociated to a single-cell suspension and replated in the selective culture medium. Removal of growth factors and plating cells on an adherent substrate allows cells to differentiate into neurons, astrocytes, and oligodendrocytes, the main cell type of the CNS. Correct culturing of NSCs, according to this methodology, will allow cells to expand over 100 passages without alteration of cell karyotype, growth ability, and differentiation potential.
AB - This unit describes protocols for the derivation, characterization, and expansion of neural stem cell (NSC) lines from the adult mouse subvetricular zone (mNSCs), embryonic mouse brain and from the human fetal brain (hNSCs). NSCs can be isolated by enzymatic digestion of specific regions (NSCs niches) of the central nervous system (CNS) and grown in suspension. By using this methodology, NSCs form spherical clusters called neuropsheres, which are mechanically dissociated to a single-cell suspension and replated in the selective culture medium. Removal of growth factors and plating cells on an adherent substrate allows cells to differentiate into neurons, astrocytes, and oligodendrocytes, the main cell type of the CNS. Correct culturing of NSCs, according to this methodology, will allow cells to expand over 100 passages without alteration of cell karyotype, growth ability, and differentiation potential.
KW - Clonal analysis
KW - Neural stem cells (NSC)
KW - Neurospheres
KW - Subventricular zone (SVZ)
UR - http://www.scopus.com/inward/record.url?scp=79953270645&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79953270645&partnerID=8YFLogxK
U2 - 10.1002/9780470151808.sc02d06s15
DO - 10.1002/9780470151808.sc02d06s15
M3 - Article
C2 - 21049474
AN - SCOPUS:79953270645
JO - Current Protocols in Stem Cell Biology
JF - Current Protocols in Stem Cell Biology
SN - 1941-7322
IS - SUPPL.15
M1 - 2D.6
ER -