Isolation of mouse respiratory epithelial cells and exposure to experimental cigarette smoke at air liquid interface

Hilaire C. Lam, Augustine M.K. Choi, Stefan W. Ryter

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Pulmonary epithelial cells can be isolated from the respiratory tract of mice and cultured at air-liquid interface (ALI) as a model of differentiated respiratory epithelium. A protocol is described for isolating and exposing these cells to mainstream cigarette smoke (CS), in order to study epithelial cell responses to CS exposure. The protocol consists of three parts: the isolation of airway epithelial cells from mouse trachea, the culturing of these cells at air-liquid interface (ALI) as fully differentiated epithelial cells, and the delivery of calibrated mainstream CS to these cells in culture. The ALI culture system allows the culture of respiratory epithelia under conditions that more closely resemble their physiological setting than ordinary liquid culture systems. The study of molecular and lung cellular responses to CS exposure is a critical component of understanding the impact of environmental air pollution on human health. Research findings in this area may ultimately contribute towards understanding the etiology of chronic obstructive pulmonary disease (COPD), and other tobacco-related diseases, which represent major global health problems.

Original languageEnglish (US)
Article numbere2513
JournalJournal of Visualized Experiments
Issue number48
DOIs
StatePublished - Feb 2011

Keywords

  • Air-Liquid Interface
  • Cell isolation
  • Cigarette smoke
  • Epithelial cells
  • Issue 48
  • Medicine

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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