Isolation of adoptively transferred CD8+ T cells in mouse tumor tissues for lipid peroxidation detection

Liuling Xiao, Qing Yi

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

The lipid peroxidation level of tumor-infiltrating CD8+ T cells is crucial for its activity and longevity. Here, we describe a protocol for effective and epitope-preserving dissociation of mouse tumors and subsequent leukocyte purification and lipid peroxidation staining of adoptively transferred CD8+ T cells. We use BODIPY 581/591 C11 to monitor the cellular lipid peroxidation level and detect its fluorescent change by flow cytometry, followed by analysis in FlowJo. This protocol is adaptable to intrinsic CD8+ T cells in tumors as well. For complete details on the use and execution of this protocol, please refer to Xiao et al. (2022)1 and Ma et al. (2021).2

Original languageEnglish (US)
Article number101945
JournalSTAR Protocols
Volume4
Issue number1
Early online dateDec 15 2022
DOIs
StatePublished - Mar 17 2023

Keywords

  • Cancer
  • Cell Biology
  • Flow Cytometry/Mass Cytometry
  • Immunology
  • Metabolism
  • Molecular/Chemical Probes
  • Single Cell

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Fingerprint

Dive into the research topics of 'Isolation of adoptively transferred CD8+ T cells in mouse tumor tissues for lipid peroxidation detection'. Together they form a unique fingerprint.

Cite this