Abstract
The lipid peroxidation level of tumor-infiltrating CD8+ T cells is crucial for its activity and longevity. Here, we describe a protocol for effective and epitope-preserving dissociation of mouse tumors and subsequent leukocyte purification and lipid peroxidation staining of adoptively transferred CD8+ T cells. We use BODIPY 581/591 C11 to monitor the cellular lipid peroxidation level and detect its fluorescent change by flow cytometry, followed by analysis in FlowJo. This protocol is adaptable to intrinsic CD8+ T cells in tumors as well. For complete details on the use and execution of this protocol, please refer to Xiao et al. (2022)1 and Ma et al. (2021).2
Original language | English (US) |
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Article number | 101945 |
Journal | STAR Protocols |
Volume | 4 |
Issue number | 1 |
DOIs | |
State | Published - Mar 17 2023 |
Keywords
- Cancer
- Cell Biology
- Flow Cytometry/Mass Cytometry
- Immunology
- Metabolism
- Molecular/Chemical Probes
- Single Cell
ASJC Scopus subject areas
- Neuroscience(all)
- Biochemistry, Genetics and Molecular Biology(all)
- Immunology and Microbiology(all)