Isolation, chemical characterization, and biophysical properties of three different abnormal lipoproteins: LP X1, LP X2, and LP X3

J. R. Patsch, K. C. Aune, A. M. Gotto, J. D. Morrisett

Research output: Contribution to journalArticlepeer-review

53 Scopus citations

Abstract

Three different but related abnormal lipoprotein species, LP X1, LP X2, and LP X3, have been isolated from cholestatic plasma by ethanol precipitation and zonal ultracentrifugation. All three populations are rich in phospholipids (64.9 to 67.5%) and cholesterol (23.0 to 26.8%) but poor in cholesteryl esters (0.4 to 1.9%), triglycerides (1.8 to 3.2%), and protein (3.2 to 6.7%) with differences in chemical composition which result in buoyant densities (1.038, 1.049, and 1.058, respectively) to allow their separation. LP X1, LP X2, and LP X3 exhibited apparent flotation rates of 17.3, 9.7, and 3.2 Svedbergs and Stokes radii of 339, 343, and 294 Å, respectively. As determined from circular dichroic measurements, the protein constituents of all three particles possessed a high degree of α helical structure (41 to 65%). Each LP X particle exhibited abnormally low fluidity as evaluated by electron paramagnetic resonance. All of the particles contained human serum albumin and the C proteins as major protein constituents, but only LP X2 and LP X3 contained apolipoprotein A I and apolipoprotein E.

Original languageEnglish (US)
Pages (from-to)2113-2120
Number of pages8
JournalJournal of Biological Chemistry
Volume252
Issue number6
StatePublished - 1977

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint

Dive into the research topics of 'Isolation, chemical characterization, and biophysical properties of three different abnormal lipoproteins: LP X<sub>1</sub>, LP X<sub>2</sub>, and LP X<sub>3</sub>'. Together they form a unique fingerprint.

Cite this