Isolation and characterization of variant benzo[A]pyrene-resistant T47D human breast-cancer cells

Michael Moore, Mary Ruh, Michael Steinberg, Stephen Safe

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

T47D human breast cancer cells were grown in 1 μM benzo[a]pyrene (BaP) for 3.5 months, and 2 BaP-resistant (BaP(R)) variant cell lines (C5 and C10) were isolated. Decreased aryl hydrocarbon (Ah)-responsiveness in the C5 and C10 BaP(R) cells was characterized by lower (80 to 90%) induction of CYP1A1-dependent activity by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), lower levels of the nuclear Ah receptor complex and significantly decreased Ah receptor mRNA levels. Nuclear estrogen receptor (ER) binding and ER mRNA levels were similar in wild-type and mutant cell lines, whereas epidermal growth factor receptor mRNA levels were significantly decreased in the variant BaP(R) T47D cells. 17β-Estradiol induced proliferation of bath wild-type and BaP(R) T47D cells, and TCDD inhibited this response but did not down-regulate nuclear ER levels. The unique characteristics of the BaP(R) T47D variant cells will be used to further elucidate the mechanism of interaction between the ER and Ah receptor signalling pathways.

Original languageEnglish (US)
Pages (from-to)117-123
Number of pages7
JournalInternational Journal of Cancer
Volume66
Issue number1
DOIs
StatePublished - Mar 28 1996
Externally publishedYes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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