Isolation and characterization of naturally occurring hairpin structures in single-stranded DNA of coliphage M13

Salil K. Niyogi, Sankar Mitra

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

With precise conditions of digestion with single-strand-specific nucleases, namely, endonuclease S1 of Aspergillus oryzae and exonuclease I of Escherichia coli, nuclease-resistant DNA cores can be obtained reproducibly from single-stranded M13 DNA. The DNA cores are composed almost exclusively of two sizes (60 and 44 nucleotides long). These have high (G + C)-contents relative to that of intact M13 DNA, and arise from restricted regions of the M13 genome. The resistance of these fragments to single-strand-specific nucleases and their nondenaturability strongly suggest the presence of double-stranded segments in these core pieces. That the core pieces are only partially double-stranded is shown by their lack of complete base complementarity and their pattern of elution from hydroxyapatite.

Original languageEnglish (US)
Pages (from-to)1037-1044
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume79
Issue number4
DOIs
StatePublished - Dec 21 1977

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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