Isolation and characterization of AINT: A novel ARNT interacting protein expressed during murine embryonic development

Christine M. Sadek, Stéphan Jalaguier, Eimear P. Feeney, Marjo Aitola, Anastasios E. Damdimopoulos, Markku Pelto-Huikko, Jan Åke Gustafsson

Research output: Contribution to journalArticlepeer-review

63 Scopus citations

Abstract

Basic helix-loop-helix-PER-ARNT-SIM (bHLH-PAS) proteins form dimeric transcription factors to mediate diverse biological functions including xenobiotic metabolism, hypoxic response, circadian rhythm and central nervous system midline development. The Ah receptor nuclear translocator protein (ARNT) plays a central role as a common heterodimerization partner. Herein, we describe a novel, embryonically expressed, ARNT interacting protein (AINT) that may be a member of a larger coiled-coil PAS interacting protein family. The AINT C-terminus mediates interaction with the PAS domain of ARNT in yeast and interacts in vitro with ARNT and ARNT2 specifically. AINT localizes to the cytoplasm and overexpression leads to non-nuclear localization of ARNT. A dynamic pattern of AINT mRNA expression during embryogenesis and cerebellum ontogeny supports a role for AINT in development. (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish (US)
Pages (from-to)13-26
Number of pages14
JournalMechanisms of Development
Volume97
Issue number1-2
DOIs
StatePublished - Oct 2000

Keywords

  • Ah receptor nuclear translocator
  • ARNT
  • ARNT2
  • Basic helix-loop-helix-PAS
  • bHLH-PAS
  • Coiled-coil
  • HIF-1α
  • HLF
  • Hypoxia
  • NPAS
  • TACC
  • Yeast two- hybrid

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

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