TY - JOUR
T1 - Is reliance on an inaccurate genome sequence sabotaging your experiments?
AU - Baptista, Rodrigo P.
AU - Kissinger, Jessica C.
N1 - Publisher Copyright:
Copyright © 2019 Baptista, Kissinger.
PY - 2019
Y1 - 2019
N2 - Advances in genomics have made whole genome studies increasingly feasible across the life sciences. However, new technologies and algorithmic advances do not guarantee flawless genomic sequences or annotation. Bias, errors, and artifacts can enter at any stage of the process from library preparation to annotation. When planning an experiment that utilizes a genome sequence as the basis for the design, there are a few basic checks that, if performed, may better inform the experimental design and ideally help avoid a failed experiment or inconclusive result.
AB - Advances in genomics have made whole genome studies increasingly feasible across the life sciences. However, new technologies and algorithmic advances do not guarantee flawless genomic sequences or annotation. Bias, errors, and artifacts can enter at any stage of the process from library preparation to annotation. When planning an experiment that utilizes a genome sequence as the basis for the design, there are a few basic checks that, if performed, may better inform the experimental design and ideally help avoid a failed experiment or inconclusive result.
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U2 - 10.1371/journal.ppat.1007901
DO - 10.1371/journal.ppat.1007901
M3 - Article
C2 - 31513692
AN - SCOPUS:85072151124
VL - 15
JO - PLoS pathogens
JF - PLoS pathogens
SN - 1553-7366
IS - 9
M1 - e1007901
ER -