IRE1α-XBP1 signaling in leukocytes controls prostaglandin biosynthesis and pain

Sahil Chopra, Paolo Giovanelli, Perla Abigail Alvarado-Vazquez, Sara Alonso, Minkyung Song, Tito A. Sandoval, Chang Suk Chae, Chen Tan, Miriam M. Fonseca, Silvia Gutierrez, Leandro Jimenez, Kotha Subbaramaiah, Takao Iwawaki, Philip J. Kingsley, Lawrence J. Marnett, Andrew V. Kossenkov, Mariano Sanchez Crespo, Andrew J. Dannenberg, Laurie H. Glimcher, E. Alfonso Romero-SandovalJuan R. Cubillos-Ruiz

Research output: Contribution to journalArticlepeer-review

33 Scopus citations


Inositol-requiring enzyme 1[α] (IRE1[α])-X-box binding protein spliced (XBP1) signalingmaintains endoplasmic reticulum (ER) homeostasis while controlling immunometabolic processes. Yet, the physiological consequences of IRE1α-XBP1 activation in leukocytes remain unexplored. We found that induction of prostaglandin-endoperoxide synthase 2 (Ptgs2/Cox-2) and prostaglandin E synthase (Ptges/mPGES-1) was compromised in IRE1α-deficient myeloid cells undergoing ER stress or stimulated through pattern recognition receptors. Inducible biosynthesis of prostaglandins, including the pro-algesic mediator prostaglandin E2 (PGE2), was decreased in myeloid cells that lack IRE1α or XBP1 but not other ER stress sensors. Functional XBP1 transactivated the human PTGS2 and PTGES genes to enable optimal PGE2 production. Mice that lack IRE1α-XBP1 in leukocytes, or that were treated with IRE1α inhibitors, demonstrated reduced pain behaviors in PGE2-dependent models of pain. Thus, IRE1α-XBP1 is a mediator of prostaglandin biosynthesis and a potential target to control pain.

Original languageEnglish (US)
Article numbereaau6499
Issue number6450
StatePublished - Jul 19 2019

ASJC Scopus subject areas

  • General


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