TY - JOUR
T1 - Involvement of PhoP-PhoS homologs in Enterococcus faecalis virulence
AU - Teng, Fang
AU - Wang, Ling
AU - Singh, Kavindra V.
AU - Murray, Barbara E.
AU - Weinstock, George M.
PY - 2002
Y1 - 2002
N2 - Eleven PhoP-PhoS homolog pairs were identified by searching the Enterococcus faecalis V583 genome sequence database at The Institute for Genomic Research with the Bacillus subtilis PhoP-PhoS sequences. Each pair appears to be a potential two-component system composed of a response regulator and a sensor kinase. Seven of the homologs were disrupted in E. faecalis strain OG1RF. TX10293, a mutant disrupted in one of these genes (etaR, the first gene of the gene pair designated etaRS), showed delayed killing and a higher 50% lethal dose in a mouse peritonitis model. The predicted EtaR protein sequence showed greatest similarity to LisR of Listeria monocytogenes (77%) and CsrR of Streptococcus pyogenes (70%); EtaS is 53% similar to LisK and 54% similar to CsrS. When grown in vitro, the TX10293 mutant was more sensitive to low pH (pH 3.4) and more resistant to high temperature (55°C) than wild-type OG1RF. In conclusion, many potential two-component systems are identified for E. faecalis, one of which, EtaRS, was shown to be involved in stress response and virulence.
AB - Eleven PhoP-PhoS homolog pairs were identified by searching the Enterococcus faecalis V583 genome sequence database at The Institute for Genomic Research with the Bacillus subtilis PhoP-PhoS sequences. Each pair appears to be a potential two-component system composed of a response regulator and a sensor kinase. Seven of the homologs were disrupted in E. faecalis strain OG1RF. TX10293, a mutant disrupted in one of these genes (etaR, the first gene of the gene pair designated etaRS), showed delayed killing and a higher 50% lethal dose in a mouse peritonitis model. The predicted EtaR protein sequence showed greatest similarity to LisR of Listeria monocytogenes (77%) and CsrR of Streptococcus pyogenes (70%); EtaS is 53% similar to LisK and 54% similar to CsrS. When grown in vitro, the TX10293 mutant was more sensitive to low pH (pH 3.4) and more resistant to high temperature (55°C) than wild-type OG1RF. In conclusion, many potential two-component systems are identified for E. faecalis, one of which, EtaRS, was shown to be involved in stress response and virulence.
UR - http://www.scopus.com/inward/record.url?scp=0036127014&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036127014&partnerID=8YFLogxK
U2 - 10.1128/IAI.70.4.1991-1996.2002
DO - 10.1128/IAI.70.4.1991-1996.2002
M3 - Article
C2 - 11895963
AN - SCOPUS:0036127014
SN - 0019-9567
VL - 70
SP - 1991
EP - 1996
JO - Infection and Immunity
JF - Infection and Immunity
IS - 4
ER -