Investigation of macrophage polarization using bone marrow derived macrophages

Wei Ying; Patali S. Cheruku; Fuller W. Bazer; Stephen H. Safe; Beiyan Zhou

doi:10.3791/50323

Investigation of macrophage polarization using bone marrow derived macrophages

Wei Ying, Patali S. Cheruku, Fuller W. Bazer, Stephen H. Safe, Beiyan Zhou

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305 Scopus citations

Abstract

The article describes a readily easy adaptive in vitro model to investigate macrophage polarization. In the presence of GM-CSF/M-CSF, hematopoietic stem/progenitor cells from the bone marrow are directed into monocytic differentiation, followed by M1 or M2 stimulation. The activation status can be tracked by changes in cell surface antigens, gene expression and cell signaling pathways.

Original language	English (US)
Journal	Journal of visualized experiments : JoVE
Issue number	76
DOIs	https://doi.org/10.3791/50323
State	Published - 2013

ASJC Scopus subject areas

General Neuroscience
General Chemical Engineering
General Biochemistry, Genetics and Molecular Biology
General Immunology and Microbiology

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10.3791/50323

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title = "Investigation of macrophage polarization using bone marrow derived macrophages",

abstract = "The article describes a readily easy adaptive in vitro model to investigate macrophage polarization. In the presence of GM-CSF/M-CSF, hematopoietic stem/progenitor cells from the bone marrow are directed into monocytic differentiation, followed by M1 or M2 stimulation. The activation status can be tracked by changes in cell surface antigens, gene expression and cell signaling pathways. ",

author = "Wei Ying and Cheruku, {Patali S.} and Bazer, {Fuller W.} and Safe, {Stephen H.} and Beiyan Zhou",

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AU - Cheruku, Patali S.

AU - Bazer, Fuller W.

AU - Safe, Stephen H.

AU - Zhou, Beiyan

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PY - 2013

Y1 - 2013

N2 - The article describes a readily easy adaptive in vitro model to investigate macrophage polarization. In the presence of GM-CSF/M-CSF, hematopoietic stem/progenitor cells from the bone marrow are directed into monocytic differentiation, followed by M1 or M2 stimulation. The activation status can be tracked by changes in cell surface antigens, gene expression and cell signaling pathways.

AB - The article describes a readily easy adaptive in vitro model to investigate macrophage polarization. In the presence of GM-CSF/M-CSF, hematopoietic stem/progenitor cells from the bone marrow are directed into monocytic differentiation, followed by M1 or M2 stimulation. The activation status can be tracked by changes in cell surface antigens, gene expression and cell signaling pathways.

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Investigation of macrophage polarization using bone marrow derived macrophages

Abstract

ASJC Scopus subject areas

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