Investigation of macrophage polarization using bone marrow derived macrophages

Wei Ying; Patali S. Cheruku; Fuller W. Bazer; Stephen H. Safe; Beiyan Zhou

doi:10.3791/50323

Investigation of macrophage polarization using bone marrow derived macrophages

Wei Ying, Patali S. Cheruku, Fuller W. Bazer, Stephen H. Safe, Beiyan Zhou

Research output: Contribution to journal › Article

119 Scopus citations

Abstract

The article describes a readily easy adaptive in vitro model to investigate macrophage polarization. In the presence of GM-CSF/M-CSF, hematopoietic stem/progenitor cells from the bone marrow are directed into monocytic differentiation, followed by M1 or M2 stimulation. The activation status can be tracked by changes in cell surface antigens, gene expression and cell signaling pathways.

Original language	English (US)
Journal	Journal of visualized experiments : JoVE
Issue number	76
DOIs	https://doi.org/10.3791/50323
State	Published - 2013

ASJC Scopus subject areas

Neuroscience(all)
Chemical Engineering(all)
Biochemistry, Genetics and Molecular Biology(all)
Immunology and Microbiology(all)

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10.3791/50323

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title = "Investigation of macrophage polarization using bone marrow derived macrophages",

abstract = "The article describes a readily easy adaptive in vitro model to investigate macrophage polarization. In the presence of GM-CSF/M-CSF, hematopoietic stem/progenitor cells from the bone marrow are directed into monocytic differentiation, followed by M1 or M2 stimulation. The activation status can be tracked by changes in cell surface antigens, gene expression and cell signaling pathways. ",

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