Investigating the stability of gold nanorods modified with thiol molecules for biosensing

Yongxiang Gong; Jian Liu; Runcong Liu; Jiaojiao Wang; Chunmei Niu; Wenfeng Zhu; Dongmei Xu; Zhongbo Hu; Min Li; Yuliang Zhao

doi:10.1039/c5ra22133a

Investigating the stability of gold nanorods modified with thiol molecules for biosensing

Yongxiang Gong, Jian Liu, Runcong Liu, Jiaojiao Wang, Chunmei Niu, Wenfeng Zhu, Dongmei Xu, Zhongbo Hu, Min Li, Yuliang Zhao

Houston Methodist

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Gold nanorods (GNRs) modified with functional molecules are useful in chemical and biological sensing. In this work, the extinction spectrum of purified GNRs prepared by seed-mediated growth is systematically characterized by UV-visible absorption spectroscopy to test its stability before and after alkane-thiol modification. The results show that purification of GNRs makes the GNRs less stable, while a GNRs colloid solution functionalized by 11-mercaptoundecanoic acid (MUA), 3,3′-dithiobis[6-nitrobenzoic acid]bis(succinimide)ester (DSNB), or 16-mercaptohexadecanoic acid (MHA), respectively is stable over time. Bovine serum albumin (BSA) as a sample protein can be successfully attached to DSNB modified GNRs and form stable BSA-DSNB-GNRs probes. The red-shift observed in the extinction spectrum due to the BSA attachment is consistent over repeated experiments. Finally, anti-PSA (prostate-specific antigen) as a capture antibody is also attached to DSNB-modified GNRs. The attached anti-PSA is capable of interacting with prostate-specific antigens and induces a further red-shift, suggesting potential applications of thiol modified GNRs in bio-sensing.

Original language	English (US)
Pages (from-to)	174-178
Number of pages	5
Journal	RSC Advances
Volume	6
Issue number	1
DOIs	https://doi.org/10.1039/c5ra22133a
State	Published - 2015

ASJC Scopus subject areas

General Chemistry
General Chemical Engineering

Access to Document

10.1039/c5ra22133a

Cite this

@article{78032d5526474e7b87f65ef22b48087d,

title = "Investigating the stability of gold nanorods modified with thiol molecules for biosensing",

abstract = "Gold nanorods (GNRs) modified with functional molecules are useful in chemical and biological sensing. In this work, the extinction spectrum of purified GNRs prepared by seed-mediated growth is systematically characterized by UV-visible absorption spectroscopy to test its stability before and after alkane-thiol modification. The results show that purification of GNRs makes the GNRs less stable, while a GNRs colloid solution functionalized by 11-mercaptoundecanoic acid (MUA), 3,3′-dithiobis[6-nitrobenzoic acid]bis(succinimide)ester (DSNB), or 16-mercaptohexadecanoic acid (MHA), respectively is stable over time. Bovine serum albumin (BSA) as a sample protein can be successfully attached to DSNB modified GNRs and form stable BSA-DSNB-GNRs probes. The red-shift observed in the extinction spectrum due to the BSA attachment is consistent over repeated experiments. Finally, anti-PSA (prostate-specific antigen) as a capture antibody is also attached to DSNB-modified GNRs. The attached anti-PSA is capable of interacting with prostate-specific antigens and induces a further red-shift, suggesting potential applications of thiol modified GNRs in bio-sensing.",

author = "Yongxiang Gong and Jian Liu and Runcong Liu and Jiaojiao Wang and Chunmei Niu and Wenfeng Zhu and Dongmei Xu and Zhongbo Hu and Min Li and Yuliang Zhao",

note = "Funding Information: This work was supported by the National Basic Research Program of China (no. 2011CB933101), National Natural Science Foundation of China (grant no. 21303208). The project was also sponsored by the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry (no. Y31Z55) and the Start-Up Funding from the Institute of High Energy Physics of the Chinese Academy of Sciences (No. 2011IHEPYJRC504). Publisher Copyright: {\textcopyright} 2016 The Royal Society of Chemistry. Copyright: Copyright 2016 Elsevier B.V., All rights reserved.",

year = "2015",

doi = "10.1039/c5ra22133a",

language = "English (US)",

volume = "6",

pages = "174--178",

journal = "RSC Advances",

issn = "2046-2069",

publisher = "Royal Society of Chemistry",

number = "1",

}

TY - JOUR

T1 - Investigating the stability of gold nanorods modified with thiol molecules for biosensing

AU - Gong, Yongxiang

AU - Liu, Jian

AU - Liu, Runcong

AU - Wang, Jiaojiao

AU - Niu, Chunmei

AU - Zhu, Wenfeng

AU - Xu, Dongmei

AU - Hu, Zhongbo

AU - Li, Min

AU - Zhao, Yuliang

N1 - Funding Information: This work was supported by the National Basic Research Program of China (no. 2011CB933101), National Natural Science Foundation of China (grant no. 21303208). The project was also sponsored by the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry (no. Y31Z55) and the Start-Up Funding from the Institute of High Energy Physics of the Chinese Academy of Sciences (No. 2011IHEPYJRC504). Publisher Copyright: © 2016 The Royal Society of Chemistry. Copyright: Copyright 2016 Elsevier B.V., All rights reserved.

PY - 2015

Y1 - 2015

N2 - Gold nanorods (GNRs) modified with functional molecules are useful in chemical and biological sensing. In this work, the extinction spectrum of purified GNRs prepared by seed-mediated growth is systematically characterized by UV-visible absorption spectroscopy to test its stability before and after alkane-thiol modification. The results show that purification of GNRs makes the GNRs less stable, while a GNRs colloid solution functionalized by 11-mercaptoundecanoic acid (MUA), 3,3′-dithiobis[6-nitrobenzoic acid]bis(succinimide)ester (DSNB), or 16-mercaptohexadecanoic acid (MHA), respectively is stable over time. Bovine serum albumin (BSA) as a sample protein can be successfully attached to DSNB modified GNRs and form stable BSA-DSNB-GNRs probes. The red-shift observed in the extinction spectrum due to the BSA attachment is consistent over repeated experiments. Finally, anti-PSA (prostate-specific antigen) as a capture antibody is also attached to DSNB-modified GNRs. The attached anti-PSA is capable of interacting with prostate-specific antigens and induces a further red-shift, suggesting potential applications of thiol modified GNRs in bio-sensing.

AB - Gold nanorods (GNRs) modified with functional molecules are useful in chemical and biological sensing. In this work, the extinction spectrum of purified GNRs prepared by seed-mediated growth is systematically characterized by UV-visible absorption spectroscopy to test its stability before and after alkane-thiol modification. The results show that purification of GNRs makes the GNRs less stable, while a GNRs colloid solution functionalized by 11-mercaptoundecanoic acid (MUA), 3,3′-dithiobis[6-nitrobenzoic acid]bis(succinimide)ester (DSNB), or 16-mercaptohexadecanoic acid (MHA), respectively is stable over time. Bovine serum albumin (BSA) as a sample protein can be successfully attached to DSNB modified GNRs and form stable BSA-DSNB-GNRs probes. The red-shift observed in the extinction spectrum due to the BSA attachment is consistent over repeated experiments. Finally, anti-PSA (prostate-specific antigen) as a capture antibody is also attached to DSNB-modified GNRs. The attached anti-PSA is capable of interacting with prostate-specific antigens and induces a further red-shift, suggesting potential applications of thiol modified GNRs in bio-sensing.

UR - http://www.scopus.com/inward/record.url?scp=84952325645&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84952325645&partnerID=8YFLogxK

U2 - 10.1039/c5ra22133a

DO - 10.1039/c5ra22133a

M3 - Article

AN - SCOPUS:84952325645

SN - 2046-2069

VL - 6

SP - 174

EP - 178

JO - RSC Advances

JF - RSC Advances

IS - 1

ER -

Investigating the stability of gold nanorods modified with thiol molecules for biosensing

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this