Abstract
Pancreatic cancers, both adenocarcinomas and endocrine tumors are characterized by varying levels of aberrant angiogenesis and fibrotic microenvironment. The difficulty to deliver drugs and treat the disease has been attributed in part to the vascular architecture and tissue/ECM density. Here we present longitudinal three-dimensional intravital imaging of vascular and tumor microenvironment remodeling in spontaneous transgenic tumors (RIP1-Tag2 insulinomas) and orthotopically injected tumors (KPC adenocarcinomas). Analysis of the data acquired in insulinomas revealed major differences in tumor blood vessel branching, fraction volume, number of branch points segments, vessel straightness and length compared to the normal tissue. The aggressive adenocarcinoma presented widespread peritumoral vascular remodeling and heterogeneous vascular distribution. Longitudinal imaging was used to acquire sequential vascular remodeling data during tumor progression. This work demonstrates the potential for using a pancreatic intravital imaging window for direct visualization of the tumor heterogenic microenvironments during tumor progression.
Original language | English (US) |
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Pages (from-to) | 2209-2217 |
Number of pages | 9 |
Journal | International Journal of Cancer |
Volume | 146 |
Issue number | 8 |
DOIs | |
State | Published - Apr 15 2020 |
Keywords
- adenocarcinoma
- insulinoma
- pancreatic cancer
- two-photon microscopy
- vasculogenesis
ASJC Scopus subject areas
- Oncology
- Cancer Research