Intravital imaging of vascular anomalies and extracellular matrix remodeling in orthotopic pancreatic tumors

Filip Bochner, Vishnu Mohan, Assaf Zinger, Ofra Golani, Avi Schroeder, Irit Sagi, Michal Neeman

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Pancreatic cancers, both adenocarcinomas and endocrine tumors are characterized by varying levels of aberrant angiogenesis and fibrotic microenvironment. The difficulty to deliver drugs and treat the disease has been attributed in part to the vascular architecture and tissue/ECM density. Here we present longitudinal three-dimensional intravital imaging of vascular and tumor microenvironment remodeling in spontaneous transgenic tumors (RIP1-Tag2 insulinomas) and orthotopically injected tumors (KPC adenocarcinomas). Analysis of the data acquired in insulinomas revealed major differences in tumor blood vessel branching, fraction volume, number of branch points segments, vessel straightness and length compared to the normal tissue. The aggressive adenocarcinoma presented widespread peritumoral vascular remodeling and heterogeneous vascular distribution. Longitudinal imaging was used to acquire sequential vascular remodeling data during tumor progression. This work demonstrates the potential for using a pancreatic intravital imaging window for direct visualization of the tumor heterogenic microenvironments during tumor progression.

Original languageEnglish (US)
Pages (from-to)2209-2217
Number of pages9
JournalInternational Journal of Cancer
Volume146
Issue number8
DOIs
StatePublished - Apr 15 2020

Keywords

  • adenocarcinoma
  • insulinoma
  • pancreatic cancer
  • two-photon microscopy
  • vasculogenesis

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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