Intracellular nanosensing and nanodelivery by PMMA nanoparticles

A. Giannetti; F. Baldini; M. Ballestri; G. Ghini; G. Giambastiani; A. Guerrini; G. Sotgiu; S. Tombelli; C. Trono; G. Tuci; G. Varchi

doi:10.1007/978-1-4614-3860-1_11

Intracellular nanosensing and nanodelivery by PMMA nanoparticles

A. Giannetti, F. Baldini, M. Ballestri, G. Ghini, G. Giambastiani, A. Guerrini, G. Sotgiu, S. Tombelli, C. Trono, G. Tuci, G. Varchi

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Scopus citations

Abstract

The delivery of fluorescent agents or probes to cells and tissues by using nanoparticles is currently receiving a growing interest because such nanodimensional structures can conveniently allow the preparation of small tools to spy at cellular mechanisms without interferences. We describe here the synthesis and characterization of fluorescently labeled cationic core-shell nanoparticles (NPs), made up of a core of polymethylmethacrylate (PMMA), surrounded by a shell bearing cationic groups, modified for nanosensing purposes. In particular, the NPs have been characterized as potential intracellular nanocarriers of molecular beacons (MB) for tumor mRNA sensing. Survivin targeting MBs have been used with Atto647N and Blackberry 650 as fluorophore/quencher pair. The MB was anchored to the surface of the PMMA nanoparticles via a commercial sulfhydryl-reactive heterobifunctional crosslinker and the achieved nanomaterials have been then characterized in vitro.

Original language	English (US)
Title of host publication	Proceedings of the First National Conference on Sensors
Pages	69-75
Number of pages	7
DOIs	https://doi.org/10.1007/978-1-4614-3860-1_11
State	Published - 2014
Event	1st National Conference on Sensors - Rome, Italy Duration: Feb 15 2012 → Feb 17 2012

Publication series

Name	Lecture Notes in Electrical Engineering
Volume	162 LNEE
ISSN (Print)	1876-1100
ISSN (Electronic)	1876-1119

Other

Other	1st National Conference on Sensors
Country/Territory	Italy
City	Rome
Period	2/15/12 → 2/17/12

ASJC Scopus subject areas

Industrial and Manufacturing Engineering

Access to Document

10.1007/978-1-4614-3860-1_11

Cite this

Giannetti, A., Baldini, F., Ballestri, M., Ghini, G., Giambastiani, G., Guerrini, A., Sotgiu, G., Tombelli, S., Trono, C., Tuci, G., & Varchi, G. (2014). Intracellular nanosensing and nanodelivery by PMMA nanoparticles. In Proceedings of the First National Conference on Sensors (pp. 69-75). (Lecture Notes in Electrical Engineering; Vol. 162 LNEE). https://doi.org/10.1007/978-1-4614-3860-1_11

Giannetti, A, Baldini, F, Ballestri, M, Ghini, G, Giambastiani, G, Guerrini, A, Sotgiu, G, Tombelli, S, Trono, C, Tuci, G & Varchi, G 2014, Intracellular nanosensing and nanodelivery by PMMA nanoparticles. in Proceedings of the First National Conference on Sensors. Lecture Notes in Electrical Engineering, vol. 162 LNEE, pp. 69-75, 1st National Conference on Sensors, Rome, Italy, 2/15/12. https://doi.org/10.1007/978-1-4614-3860-1_11

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abstract = "The delivery of fluorescent agents or probes to cells and tissues by using nanoparticles is currently receiving a growing interest because such nanodimensional structures can conveniently allow the preparation of small tools to spy at cellular mechanisms without interferences. We describe here the synthesis and characterization of fluorescently labeled cationic core-shell nanoparticles (NPs), made up of a core of polymethylmethacrylate (PMMA), surrounded by a shell bearing cationic groups, modified for nanosensing purposes. In particular, the NPs have been characterized as potential intracellular nanocarriers of molecular beacons (MB) for tumor mRNA sensing. Survivin targeting MBs have been used with Atto647N and Blackberry 650 as fluorophore/quencher pair. The MB was anchored to the surface of the PMMA nanoparticles via a commercial sulfhydryl-reactive heterobifunctional crosslinker and the achieved nanomaterials have been then characterized in vitro.",

author = "A. Giannetti and F. Baldini and M. Ballestri and G. Ghini and G. Giambastiani and A. Guerrini and G. Sotgiu and S. Tombelli and C. Trono and G. Tuci and G. Varchi",

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AU - Giannetti, A.

AU - Baldini, F.

AU - Ballestri, M.

AU - Ghini, G.

AU - Giambastiani, G.

AU - Guerrini, A.

AU - Sotgiu, G.

AU - Tombelli, S.

AU - Trono, C.

AU - Tuci, G.

AU - Varchi, G.

PY - 2014

Y1 - 2014

N2 - The delivery of fluorescent agents or probes to cells and tissues by using nanoparticles is currently receiving a growing interest because such nanodimensional structures can conveniently allow the preparation of small tools to spy at cellular mechanisms without interferences. We describe here the synthesis and characterization of fluorescently labeled cationic core-shell nanoparticles (NPs), made up of a core of polymethylmethacrylate (PMMA), surrounded by a shell bearing cationic groups, modified for nanosensing purposes. In particular, the NPs have been characterized as potential intracellular nanocarriers of molecular beacons (MB) for tumor mRNA sensing. Survivin targeting MBs have been used with Atto647N and Blackberry 650 as fluorophore/quencher pair. The MB was anchored to the surface of the PMMA nanoparticles via a commercial sulfhydryl-reactive heterobifunctional crosslinker and the achieved nanomaterials have been then characterized in vitro.

AB - The delivery of fluorescent agents or probes to cells and tissues by using nanoparticles is currently receiving a growing interest because such nanodimensional structures can conveniently allow the preparation of small tools to spy at cellular mechanisms without interferences. We describe here the synthesis and characterization of fluorescently labeled cationic core-shell nanoparticles (NPs), made up of a core of polymethylmethacrylate (PMMA), surrounded by a shell bearing cationic groups, modified for nanosensing purposes. In particular, the NPs have been characterized as potential intracellular nanocarriers of molecular beacons (MB) for tumor mRNA sensing. Survivin targeting MBs have been used with Atto647N and Blackberry 650 as fluorophore/quencher pair. The MB was anchored to the surface of the PMMA nanoparticles via a commercial sulfhydryl-reactive heterobifunctional crosslinker and the achieved nanomaterials have been then characterized in vitro.

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T3 - Lecture Notes in Electrical Engineering

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EP - 75

BT - Proceedings of the First National Conference on Sensors

T2 - 1st National Conference on Sensors

Y2 - 15 February 2012 through 17 February 2012

ER -

Intracellular nanosensing and nanodelivery by PMMA nanoparticles

Abstract

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