Intracellular delivery of molecular beacons by PMMA nanoparticles and carbon nanotubes for mRNA sensing

A. Giannetti, S. Tombelli, C. Trono, M. Ballestri, G. Giambastiani, A. Guerrini, G. Sotgiu, G. Tuci, Greta Varchi, F. Baldini

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Scopus citations

Abstract

We describe here the use of carbon nanotubes (CNTs) and polymeric nanoparticles made of a core of polymethylmethacrylate (PMMA) surrounded by a shell bearing cationic groups, as intracellular delivery tools of molecular beacons (MBs), particular fluorescent DNA probes, for the detection and localization of a specific mRNA. Survivin mRNA targeting MBs have been used with Atto647N and Blackberry 650 as fluorophore/quencher pair. The MB was anchored to the surface of CNTs and PMMA nanoparticles via a commercial sulfhydryl-reactive heterobifunctional crosslinker and the achieved nanomaterials were then characterized in vitro.

Original languageEnglish (US)
Title of host publicationReporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications V
Volume8596
DOIs
StatePublished - May 30 2013
EventReporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications V - San Francisco, CA, United States
Duration: Feb 4 2013Feb 6 2013

Other

OtherReporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications V
CountryUnited States
CitySan Francisco, CA
Period2/4/132/6/13

Keywords

  • Molecular beacon
  • carbon nanotubes
  • intracellular delivery
  • intracellular sensing
  • polymethylmethacrylate nanoparticles
  • survivin

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Fingerprint

Dive into the research topics of 'Intracellular delivery of molecular beacons by PMMA nanoparticles and carbon nanotubes for mRNA sensing'. Together they form a unique fingerprint.

Cite this