Internalization by PMMA nanoparticle-mediated endocytosis of a survivin molecular beacon as theranostic agent in human cancer cells.

B. Adinolfi; S. Tombelli; C. Trono; A. Giannetti; M. Pellegrino; G. Sotgiu; Greta Varchi; M. Ballestri; F. Baldini

doi:10.1117/12.2547604

Internalization by PMMA nanoparticle-mediated endocytosis of a survivin molecular beacon as theranostic agent in human cancer cells.

B. Adinolfi, S. Tombelli, C. Trono, A. Giannetti, M. Pellegrino, G. Sotgiu, Greta Varchi, M. Ballestri, F. Baldini

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Scopus citations

Abstract

One of the main goals in nanomedicine is the development of effective drug delivery systems. Polymeric nanoparticles (NPs) have been exploited as nanocarriers for their stability, handiness and biocompatibility. In this context, NPs have been coupled to molecular beacons (MBs), theranostic agents that conjugate the sensing of specific mRNA with a silencing activity. The aim of this work was to evaluate by confocal microscopy and fluorescence measurements: a) the involvement of endocytosis in the NP uptake; b) the NP fate at different times of cell incubation to verify their localization in lysosomes; c) the MB localization on the Endoplasmic Reticulum (ER) where the target mRNA is located. Results: a) PMMA-NPs promote endocytosis; b) strong co-localization of NP and lysosome tracker fluorescence after 2- 48 hours of incubation; c) co-localization of the MB fluorescence with the ER-marker signal after 90' of incubation. The data highlight the ability of the PMMA-NPs to promote the survivin-MB internalization by endocytosis and demonstrate that the PMMA-NPs are an appropriate delivery system capable of being eliminated by cells; these evidences also contribute to consider the MB as an effective tool for the intracellular sensing.

Original language	English (US)
Title of host publication	Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVII
Editors	Dror Fixler, Ewa M. Goldys, Sebastian Wachsmann-Hogiu
Publisher	SPIE
Volume	11254
ISBN (Electronic)	9781510632714
DOIs	https://doi.org/10.1117/12.2547604
State	Published - Jan 1 2020
Event	Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVII 2020 - San Francisco, United States Duration: Feb 2 2020 → Feb 3 2020

Other

Other	Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVII 2020
Country/Territory	United States
City	San Francisco
Period	2/2/20 → 2/3/20

Keywords

Cancer cells
Endocytosis
Lysosomal exocytosis
Molecular beacon
Polymethylmethacrylate nanoparticles

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Radiology Nuclear Medicine and imaging

Access to Document

10.1117/12.2547604

Cite this

Adinolfi, B., Tombelli, S., Trono, C., Giannetti, A., Pellegrino, M., Sotgiu, G., Varchi, G., Ballestri, M., & Baldini, F. (2020). Internalization by PMMA nanoparticle-mediated endocytosis of a survivin molecular beacon as theranostic agent in human cancer cells. In D. Fixler, E. M. Goldys, & S. Wachsmann-Hogiu (Eds.), Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVII (Vol. 11254). Article 112540H SPIE. https://doi.org/10.1117/12.2547604

Internalization by PMMA nanoparticle-mediated endocytosis of a survivin molecular beacon as theranostic agent in human cancer cells. / Adinolfi, B.; Tombelli, S.; Trono, C. et al.
Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVII. ed. / Dror Fixler; Ewa M. Goldys; Sebastian Wachsmann-Hogiu. Vol. 11254 SPIE, 2020. 112540H.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Adinolfi, B, Tombelli, S, Trono, C, Giannetti, A, Pellegrino, M, Sotgiu, G, Varchi, G, Ballestri, M & Baldini, F 2020, Internalization by PMMA nanoparticle-mediated endocytosis of a survivin molecular beacon as theranostic agent in human cancer cells. in D Fixler, EM Goldys & S Wachsmann-Hogiu (eds), Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVII. vol. 11254, 112540H, SPIE, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVII 2020, San Francisco, United States, 2/2/20. https://doi.org/10.1117/12.2547604

Adinolfi B, Tombelli S, Trono C, Giannetti A, Pellegrino M, Sotgiu G et al. Internalization by PMMA nanoparticle-mediated endocytosis of a survivin molecular beacon as theranostic agent in human cancer cells. In Fixler D, Goldys EM, Wachsmann-Hogiu S, editors, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVII. Vol. 11254. SPIE. 2020. 112540H doi: 10.1117/12.2547604

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Internalization by PMMA nanoparticle-mediated endocytosis of a survivin molecular beacon as theranostic agent in human cancer cells.

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