Interleukin 4, but not interleukin 5 or eosinophils, is required in a murine model of acute airway hyperreactivity

David B. Corry, Hans G. Folkesson, Martha L. Warnock, David J. Erle, Michael A. Matthay, Jeanine P. Wiener-Kronish, Richard M. Locksley

Research output: Contribution to journalArticle

652 Scopus citations

Abstract

Reversible airway hyperreactivity underlies the pathophysiology of asthma, yet the precise mediators of the response remain unclear. Human studies have correlated aberrant activation of T helper (Th) 2-like effector systems in the airways with disease. A murine model of airway hyperreactivity in response to acetylcholine was established using mice immunized with ovalbumin and challenged with aerosolized antigen. No airway hyperreactivity occurred in severe combined immunodeficient mice. Identically immunized BALB/c mice developed an influx of cells, with a predominance of eosinophils and CD4+ T cells, into the lungs and bronchoalveolar lavage fluid at the time that substantial changes in airway pressure and resistance were quantitated. Challenged animals developed marked increases in Th2 cytokine production, eosinophil influx, and serum immunoglobulin E levels. Neutralization of interleukin (IL) 4 using monoclonal antibodies administered during the period of systemic immunization abrogated airway hyperreactivity but had little effect on the influx of eosinophils. Administration of anti-IL-4 only during the period of the aerosol challenge did not affect the subsequent response to acetylcholine. Finally, administration of anti-IL-5 antibodies at levels that suppressed eosinophils to <1% of recruited cells had no effect on the subsequent airway responses. BALB/c mice had significantly greater airway responses than C57BL/6 mice, consistent with enhanced IL-4 responses to antigen in BALB/c mice. Taken together, these data implicate IL-4 generated during the period of lymphocyte priming with antigen in establishing the cascade of responses required to generate airway hyperreactivity to inhaled antigen. No role for IL-5 or eosinophils could be demonstrated.

Original languageEnglish (US)
Pages (from-to)109-117
Number of pages9
JournalJournal of Experimental Medicine
Volume183
Issue number1
DOIs
StatePublished - Jan 1 1996

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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