TY - JOUR
T1 - Intercalation of Bioactive Molecules into Nanosized ZnAl Hydrotalcites for Combined Chemo and Photo Cancer Treatment
AU - Martini, Cecilia
AU - Ferroni, Claudia
AU - Gariboldi, Marzia Bruna
AU - Donnadio, Anna
AU - Aluigi, Annalisa
AU - Sotgiu, Giovanna
AU - Liscio, Fabiola
AU - Dambruoso, Paolo
AU - Navacchia, Maria Luisa
AU - Posati, Tamara
AU - Varchi, Greta
PY - 2018/11/26
Y1 - 2018/11/26
N2 - Hydrotalcites-like compounds, also known as anionic clays or layered double hydroxides, represent the only example of lamellar solid with positively charged layers and exchangeable interlayer anions. In this study, a nanostructured HTlc with formula [Zn0.72Al0.28(OH)2] Br0.28·0.69 H2O was used as inorganic drug delivery system for anticancer therapy. Two different molecules were selected for being separately intercalated into ZnAl-HTlc: the anticancer drug norcantharidin, known for inducing cell cycle arrest at G2/M phase, and the tetra-sulfonated aluminum phthalocyanine, a photosensitizer used in anticancer photodynamic therapy. The obtained hybrid ZnAl-HTlc, were characterized in terms of X-ray powder diffraction pattern, thermogravimetric analysis, scanning electron microscopy, drug release profile, in vitro cytotoxicity, and ability to produce ROS and 1O2 upon light irradiation. Our results clearly indicate that the two selected compounds are efficiently intercalated within HTlc layers. Moreover, in vitro preliminary studies on a panel of cancer cells lines account for a greater cytotoxicity of the two drugs once loaded on HTlc either when administrated singularly or in combination. In addition, the analysis of the synergistic effect of the two formulations was evaluated by determining their combination index, which showed a greater cytotoxicity when using as a 1:2 ratio of AlPcS4@HTlc and NCTD@HTlc, respectively.
AB - Hydrotalcites-like compounds, also known as anionic clays or layered double hydroxides, represent the only example of lamellar solid with positively charged layers and exchangeable interlayer anions. In this study, a nanostructured HTlc with formula [Zn0.72Al0.28(OH)2] Br0.28·0.69 H2O was used as inorganic drug delivery system for anticancer therapy. Two different molecules were selected for being separately intercalated into ZnAl-HTlc: the anticancer drug norcantharidin, known for inducing cell cycle arrest at G2/M phase, and the tetra-sulfonated aluminum phthalocyanine, a photosensitizer used in anticancer photodynamic therapy. The obtained hybrid ZnAl-HTlc, were characterized in terms of X-ray powder diffraction pattern, thermogravimetric analysis, scanning electron microscopy, drug release profile, in vitro cytotoxicity, and ability to produce ROS and 1O2 upon light irradiation. Our results clearly indicate that the two selected compounds are efficiently intercalated within HTlc layers. Moreover, in vitro preliminary studies on a panel of cancer cells lines account for a greater cytotoxicity of the two drugs once loaded on HTlc either when administrated singularly or in combination. In addition, the analysis of the synergistic effect of the two formulations was evaluated by determining their combination index, which showed a greater cytotoxicity when using as a 1:2 ratio of AlPcS4@HTlc and NCTD@HTlc, respectively.
KW - cancer cells
KW - hydrotalcites (HTlc)
KW - norcantharidin (NCTD)
KW - photodynamic therapy (PDT)
KW - synergistic effect
KW - tetra-sulfonated aluminum phthalocyanine (AlPcS4)
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U2 - 10.1021/acsanm.8b01601
DO - 10.1021/acsanm.8b01601
M3 - Article
AN - SCOPUS:85078393365
VL - 1
SP - 6387
EP - 6397
JO - ACS Applied Nano Materials
JF - ACS Applied Nano Materials
SN - 2574-0970
IS - 11
ER -