Interaction of apoliprotein C-III with phosphatidylcholine vesicles. Dependence of aproprotein-phospholipid complex formation on vesicle structure

Joel D. Morrisett, Henry J. Pownall, Antonio Gotto

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

We have studied the interaction of an apolipoprotein from human very low density lipoproteins (apoC-III) with egg yolk phosphatidylcholine in the form of single- and multi-bilayer vesicles. The reactivity of single-bilayer vesicles with apoC-III1 appears to be greater than that of the multi-bilayer vesicles according to several thermodynamic and spectroscopic criteria. In the complexes formed by the association of apoC-III with single-bilayer vesicles, the α-helical content of the peptide backbone and the apolarity of the environment around the tryptophan residues are greater than that observed in the complexes formed with the multibilayer vesicles. A higher yield and more homogeneous density distribution of lipid-apoprotein complexes results from the interaction of apoC-with the single-bilayer vesicles relative to those obtained with the multibilayer vesicles. The enthalpy of association of apoC-III1 with phospholipid was greater for the single-shelled vesicles (25 kcal/mol apoC-III1) than for the multishelled ones (18 kcal/mol apoC-III1). The difference in reactivity of these two types of liposomes is not due to a difference in their fluidities since their fatty acid compositions are identical, but may be due to a difference in their areas of sterically accessible phospholipid, their permeabilities to the apoprotein, their radii of curvation, or a combination of these factors.

Original languageEnglish (US)
Pages (from-to)36-46
Number of pages11
JournalBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume486
Issue number1
DOIs
StatePublished - Jan 18 1977

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Endocrinology

Fingerprint Dive into the research topics of 'Interaction of apoliprotein C-III with phosphatidylcholine vesicles. Dependence of aproprotein-phospholipid complex formation on vesicle structure'. Together they form a unique fingerprint.

Cite this