Integrin-targeted stabilized nanoparticles for an efficient delivery of siRNAs in vitro and in vivo

Charudharshini Srinivasan, Dan Peer, Motomu Shimaoka

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Scopus citations

Abstract

Utilizing small interfering RNAs (siRNAs) to silence disease-associated genes holds promise as a potential therapeutic strategy. However, the greatest challenge for RNAi remains the delivery of siRNA to target tissues or cells. Specifically lymphocytes are difficult to transduce by conventional methods but represent good targets for anti-inflammatory therapeutics. Integrins are an important class of cell adhesion receptors on leukocytes. Antibodies to integrins have been used to inhibit inflammatory reactions in patients. Here, we describe a strategy to deliver the siRNA cargo to leukocytes by stabilized nanoparticles surface-decorated with antibodies to integrin as targeting moieties. A detailed methodology for preparation of the integrin-targeted stabilized nanoparticles (I-tsNPs) and their delivery in vitro and in vivo is discussed.

Original languageEnglish (US)
Title of host publicationCytokine Protocols
EditorsMarc Ley
Pages105-116
Number of pages12
DOIs
StatePublished - Jan 2 2012

Publication series

NameMethods in Molecular Biology
Volume820
ISSN (Print)1064-3745

Keywords

  • Antibody
  • Hyaluronan
  • Inflammation
  • Leukocytes
  • Liposomes
  • RNAi
  • Systemic delivery
  • Transfection

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Integrin-targeted stabilized nanoparticles for an efficient delivery of siRNAs in vitro and in vivo'. Together they form a unique fingerprint.

Cite this