Integration of a bio-chip technique with technetium-99m labeling provides zeptomolar sensitivity in liver cancer biomarker detection

L. Ma, B. C. Tang, W. J. Yang, Y. Liu, Y. L. Zhao, M. Li

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

In the present work, we integrated a prepared bio-chip with 99mTc labeling to improve the immunoassay for cancer biomarker protein detection. Efficient binding of a 99mTc isotope-labeled secondary antibody to the biomarker protein pre-anchored to the bio-chip was followed by detection of γ rays from 99mTc decay via a Gamma Well Counter. We show that alpha-fetoprotein (AFP), a biomarker of liver cancer, can be detected down to 10-18 M concentration in a buffer solution with good reproducibility. This ultra-high sensitivity is attributed to the aggregation of the 99mTc isotope-labeled secondary antibody which greatly amplifies the γ rays emitted per AFP binding event. The tendency of the isotope-labeled secondary antibody to aggregate was indicated by dynamic light scattering studies on a rhenium/secondary antibody conjugate, a stable structural analogue of the 99mTc conjugate. The successful zeptomolar-range detection of cancer biomarkers opens new avenues for early disease diagnoses via integrating bio-chips with isotope-labeling techniques.

Original languageEnglish (US)
Pages (from-to)1622-1626
Number of pages5
JournalAnalytical Methods
Volume7
Issue number4
DOIs
StatePublished - Feb 21 2015

ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemical Engineering(all)
  • Engineering(all)

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