Integrating multi-scale blob/curvilinear detector techniques and multilevel sets for automated segmentation of stem cell images

Huiming Peng, Xiaobo Zhou, Fuhai Li, Xiaofeng Xia, Stephen T.C. Wong

Research output: Chapter in Book/Report/Conference proceedingConference contribution

17 Scopus citations

Abstract

Studies of differentiation abilities of stem cells have been attracting a lot of attention over the last years. Microscopy can be used to record details of the differentiation process of stem cells under different perturbations and is an important tool for studying stem cell differentiation. Since it is infeasible to quantitatively analyze a huge amount of image data manually, automated image analysis systems are urgently needed. However, the complicated morphological appearances of stem cells are challenging to the existing segmentation methods. Herein, we propose a new, automated scheme for stem cell segmentation. This scheme first uses the multi-scale blob and curvilinear structure detectors to delineate the skeletons of stem cells quickly and then segment out stem cells by refining the skeletons to the cell boundaries using multi-level sets. The initial experimental results indicate the effectiveness of the proposed scheme.

Original languageEnglish (US)
Title of host publicationProceedings - 2009 IEEE International Symposium on Biomedical Imaging
Subtitle of host publicationFrom Nano to Macro, ISBI 2009
Pages1362-1365
Number of pages4
DOIs
StatePublished - 2009
Event2009 IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2009 - Boston, MA, United States
Duration: Jun 28 2009Jul 1 2009

Publication series

NameProceedings - 2009 IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2009

Other

Other2009 IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2009
Country/TerritoryUnited States
CityBoston, MA
Period6/28/097/1/09

Keywords

  • Blob detection
  • Cell segmentation
  • Curvilinear structure detection
  • Level set
  • Stem cell differentiation

ASJC Scopus subject areas

  • Biomedical Engineering
  • Radiology Nuclear Medicine and imaging

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