Integrated Microfluidic System for Gene Silencing and Cell Migration

Zongbin Liu, Xin Han, Qing Zhou, Rui Chen, Shelby Fruge, Myeong Chan Jo, Yuan Ma, Ziyin Li, Kenji Yokoi, Lidong Qin

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Metastasis involves the phenotype transition of cancer cells to gain invasiveness, and the following migration at the tumor site. Here an integrated microfluidic chip to study this process is presented by combining on-chip delivery of siRNA for gene silencing and cell migration assay. The major advantage of the integrated chip is the simple input of cells and gene transfection materials, and the ultimate output of migration ability. The reverse-fishbone structure and 0.7× phosphate-buffered saline solution are the optimized parameters for improved delivery efficiency. Using the chip, it is validated that cofilin plays an essential role in regulating cancer cell migration. The integrated chip may provide a simple and effective platform for biologists to easily check the role of specific genes in metastasis.

Original languageEnglish (US)
Article number1700054
JournalAdvanced Biosystems
Volume1
Issue number6
DOIs
StatePublished - Jun 2017

Keywords

  • cancer cell migration
  • gene silencing
  • metastasis
  • microfluidics
  • siRNA

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Biochemistry, Genetics and Molecular Biology(all)

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