Inhibition of the 17β-estradiol-induced and constitutive expression of the cellular protooncogene c-fos by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in the female rat uterus

B. Astroff, B. Eldridge, S. Safe

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

Acute administration of 17β-estradiol (5 μg/rat) to 25-day-old female Sprague-Dawley rats resulted in an increase of uterine mRNA for the cellular oncogene c-fos. The c-fos mRNA levels were significantly elevated 12 and 24 h after exposure to the hormone (232 and 164% of control values) and the elevation was not observed after 48 h. In contrast, treatment of the animals with either 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) or 6-methyl-1,3,8-trichlorodibenzofuran (MCDF) resulted in a dose-dependent decrease in constitutive uterine c-fos mRNA levels. In rats co-treated with 17β-estradiol plus TCDD or MCDF, it was apparent from the results that the halogenated aromatic hydrocarbons significantly inhibited the estrogen-induced increases in uterine c-fos mRNA levels. These observations further extend the diverse spectrum of antiestrogenic effects caused by TCDD and related compounds and also show an interaction between TCDD and the constitutive expression of the c-fos protooncogene in the female rat uterus.

Original languageEnglish (US)
Pages (from-to)305-315
Number of pages11
JournalToxicology Letters
Volume56
Issue number3
DOIs
StatePublished - May 1991

Keywords

  • 17β-Estradiol
  • 2,3,7,8-TCDD
  • Antiestrogen
  • Rat uterus
  • c-fos mRNA induction

ASJC Scopus subject areas

  • Toxicology

Fingerprint Dive into the research topics of 'Inhibition of the 17β-estradiol-induced and constitutive expression of the cellular protooncogene c-fos by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in the female rat uterus'. Together they form a unique fingerprint.

Cite this