Inhibition of Enterococcus faecium adherence to collagen by antibodies against high-affinity binding subdomains of Acm

Sreedhar R. Nallapareddy, Jouko Sillanpää, Vannakambadi K. Ganesh, Magnus Höök, Barbara E. Murray

    Research output: Contribution to journalArticlepeer-review

    20 Scopus citations

    Abstract

    Strains of Enterococcus faecium express a cell wall-anchored protein, Acm, which mediates adherence to collagen. Here, we (i) identify the minimal and high-affinity binding subsegments of Acm and (ii) show that anti-Acm immunoglobulin Gs (IgGs) purified against these subsegments reduced E. faecium TX2535 strain collagen adherence up to 73 and 50%, respectively, significantly more than the total IgGs against the full-length Acm A domain (28%) (P < 0.0001). Blocking Acm adherence with functional subsegment-specific antibodies raises the possibility of their use as therapeutic or prophylactic agents.

    Original languageEnglish (US)
    Pages (from-to)3192-3196
    Number of pages5
    JournalInfection and Immunity
    Volume75
    Issue number6
    DOIs
    StatePublished - Jun 2007

    ASJC Scopus subject areas

    • Parasitology
    • Microbiology
    • Immunology
    • Infectious Diseases

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