TY - JOUR
T1 - Inhibition of Enterococcus faecium adherence to collagen by antibodies against high-affinity binding subdomains of Acm
AU - Nallapareddy, Sreedhar R.
AU - Sillanpää, Jouko
AU - Ganesh, Vannakambadi K.
AU - Höök, Magnus
AU - Murray, Barbara E.
PY - 2007/6
Y1 - 2007/6
N2 - Strains of Enterococcus faecium express a cell wall-anchored protein, Acm, which mediates adherence to collagen. Here, we (i) identify the minimal and high-affinity binding subsegments of Acm and (ii) show that anti-Acm immunoglobulin Gs (IgGs) purified against these subsegments reduced E. faecium TX2535 strain collagen adherence up to 73 and 50%, respectively, significantly more than the total IgGs against the full-length Acm A domain (28%) (P < 0.0001). Blocking Acm adherence with functional subsegment-specific antibodies raises the possibility of their use as therapeutic or prophylactic agents.
AB - Strains of Enterococcus faecium express a cell wall-anchored protein, Acm, which mediates adherence to collagen. Here, we (i) identify the minimal and high-affinity binding subsegments of Acm and (ii) show that anti-Acm immunoglobulin Gs (IgGs) purified against these subsegments reduced E. faecium TX2535 strain collagen adherence up to 73 and 50%, respectively, significantly more than the total IgGs against the full-length Acm A domain (28%) (P < 0.0001). Blocking Acm adherence with functional subsegment-specific antibodies raises the possibility of their use as therapeutic or prophylactic agents.
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U2 - 10.1128/IAI.02016-06
DO - 10.1128/IAI.02016-06
M3 - Article
C2 - 17438036
AN - SCOPUS:34249903609
VL - 75
SP - 3192
EP - 3196
JO - Infection and Immunity
JF - Infection and Immunity
SN - 0019-9567
IS - 6
ER -