Inhibition of Enterococcus faecium adherence to collagen by antibodies against high-affinity binding subdomains of Acm

Sreedhar R. Nallapareddy, Jouko Sillanpää, Vannakambadi K. Ganesh, Magnus Höök, Barbara E. Murray

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Strains of Enterococcus faecium express a cell wall-anchored protein, Acm, which mediates adherence to collagen. Here, we (i) identify the minimal and high-affinity binding subsegments of Acm and (ii) show that anti-Acm immunoglobulin Gs (IgGs) purified against these subsegments reduced E. faecium TX2535 strain collagen adherence up to 73 and 50%, respectively, significantly more than the total IgGs against the full-length Acm A domain (28%) (P < 0.0001). Blocking Acm adherence with functional subsegment-specific antibodies raises the possibility of their use as therapeutic or prophylactic agents.

Original languageEnglish (US)
Pages (from-to)3192-3196
Number of pages5
JournalInfection and Immunity
Volume75
Issue number6
DOIs
StatePublished - Jun 2007

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

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