Inhibition of bladder tumor growth by 1,1-bis(3′-indolyl)-1-(p- substitutedphenyl)methanes: A new class of peroxisome proliferator-activated receptor γ agonists

Wassim Kassouf, Sudhakar Chintharlapalli, Maen Abdelrahim, Gina Nelkin, Stephen Safe, Ashish M. Kamat

Research output: Contribution to journalArticle

83 Scopus citations

Abstract

1,1-Bis(3′-indolyl)-1-(p-substitutedphenyl)methanes containing p-trifluoromethyl (DIM-C-pPhCF3), p-t-butyl (DIM-C-pPhtBu), and phenyl (DIM-C-pPhC6H5) substituents have been identified as a new class of peroxisome proliferator-activated receptor γ (PPARγ) agonists that exhibit antitumorigenic activity. The PPARγ-active C-DIMs have not previously been studied against bladder cancer. We investigated the effects of the PPARγ-active C-DIMs on bladder cancer cells in vitro and bladder tumors in vivo. In this study, the PPARγ-active compounds inhibited the proliferation of KU7 and 253J-BV bladder cancer cells, and the corresponding IC50 values were 5 to 10 and 1 to 5 μmol/L, respectively. In the less responsive KU7 cells, the PPARγ agonists induced caveolin-1 and p21 expression but no changes in cyclin D1 or p27; in 253J-BV cells, the PPARγ agonists did not affect caveolin-1, cyclin D1, or p27 expression but induced p21 protein. In KU7 cells, induction of caveolin-1 by each of the PPARγ agonists was significantly down-regulated after cotreatment with the PPARγ antagonist GW9662. DIM-C-pPhCF3 (60 mg/kg thrice a week for 4 weeks) inhibited the growth of implanted KU7 orthotopic and s.c. tumors by 32% and 60%, respectively, and produced a corresponding decrease in proliferation index. Treatment of KU7 cells with DIM-C-pPhCF3 also elevated caveolin-1 expression by 25% to 30%, suggesting a role for this protein in mediating the antitumorigenic activity of DIM-C-pPhCF3 in bladder cancer.

Original languageEnglish (US)
Pages (from-to)412-418
Number of pages7
JournalCancer research
Volume66
Issue number1
DOIs
StatePublished - Jan 1 2006

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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