This study investigated the effects of continuously inhibiting nitric oxide (NO) generation during short term exercise on the physiologic parameters; work performance to exhaustion and maximum oxygen uptake (VO2max)' and on the biochemical parameters; skeletal muscle citrate synthase activity and endothelial cell nitric oxide synthase activity (cNOS). Methods: 15 10 week old female C57BL/6 mice fed a high cholesterol diet were divided into 3 groups; sedentary (SD), exercise (EX) and exercise with L-nitroarginine supplemented drinking water (6mg/100cc). (LNEX). Both exercise groups underwent a 4 week period of 1 hour twice daily treadmill running at a final speed of 22 m/min. Five days before the end of the exercise period, the L-nitroarginine supplementation was discontinued. At the end of the exercise period, VO2max was determined utilizing a metabolic chamber enclosed treadmill. At sacrifice, hindleg muscles were removed for citrate synthase assay and the abdominal aorta was removed for stimulated NO assay. Results: Inhibiting NO generation during exercise training diminishes the work performance to exhaustion and citrate synthase upregulation while it completely prevents the improvement in VO2max- Upregulation of cNOS appears unaffected. We conclude that some of the basic adaptations to exercise require normal generation of NO.
|Original language||English (US)|
|Journal||Journal of Investigative Medicine|
|State||Published - 1996|
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)