Influenza virus genome C4 promoter/origin attenuates its transcription and replication activity by the low polymerase recognition activity

Hongbing Jiang, Shijian Zhang, Qiang Wang, Jinlan Wang, Liqing Geng, Tetsuya Toyoda

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

A natural variation is observed at position 4 of the 3' -end of influenza A virus genomes, where U (U4) or C (C4) is present. The replicon activity of C4 was 28% of U4. We compared the transcription and replication activity of U4 [v84(U4)], C4 [v84(C4)] and the complimentary RNA (c84) using the purified influenza virus RNA polymerase in vitro. ApG-primed replication activities of v84(C4) and c84 were 23.8% and 7.8% of v84(U4). Globin mRNA-primed transcription activities of v84(C4) and c84 were 36.9% and 6.81% of v84(U4). De novo replication activities of v84(C4) and c84 were 21.3 and 10.2% of v84(U4). This difference came from their polymerase binding activity. When all the eight genome segments of WSN strain were changed to U4, the virus titer was 760 times higher than the wild type. However, its pathogenicity in mice was lower than the wild type.

Original languageEnglish (US)
Pages (from-to)190-196
Number of pages7
JournalVirology
Volume408
Issue number2
DOIs
StatePublished - Dec 20 2010

Keywords

  • Influenza virus
  • Interferon
  • NS1
  • RNA polymerase
  • Replication origin
  • Transcription promoter

ASJC Scopus subject areas

  • Virology

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