@article{871903aee8a8445dbd0a85bc021c3e5b,
title = "Influence of SHH/GLI1 axis on EMT mediated migration and invasion of breast cancer cells",
abstract = "Sonic Hedgehog signaling is critical for breast morphogenesis and cancer. The present study was conducted to explore the influence of SHH/GLI1 axis on epithelial mesenchymal transition and invasion in breast cancer cells. SHH/GLI1 positive samples demonstrated high expression of Snail and Vimentin with relatively low expression of E-cadherin. Overexpression of Vimentin and Snail in SHH/GLI1 positive patients was also associated with poor overall survival. Interestingly, GANT61 (GLI1 inhibitor) exposure significantly reduced cell viability and induced apoptosis at 10 µM. Suppression of Hedgehog pathway either by CRISPR mediated SHH knock out or GANT61 altered regulation of EMT markers in breast cancer cells. Moreover, in-activation of SHH/GLI1 axis also significantly restricted cell migration and invasiveness. These findings suggest that targeting SHH/GLI1 axis alters expression of EMT markers and abrogates neoplastic invasion in breast cancer cells.",
author = "Riaz, {Syeda Kiran} and Yuepeng Ke and Fen Wang and Kayani, {Mahmood Akhtar} and Malik, {Muhammad Faraz Arshad}",
note = "Funding Information: We are grateful to both national and international collaborators/participants involved in this study. We would like to show our gratitude to the members in Prof. Dr. Wang{\textquoteright}s lab especially Junchen Liu, Yi Liang and Zhiduan Cai for scientific assistance and support. We would also like to thank Leoncio Vergara (Research Scientist at Centre for Advanced Imaging, IBT) for assisting with confocal imaging. We thank Dr. Margie Moczygemba (Core Director, Center for Infectious and Inflammatory Diseases, IBT) for assisting with flow cytometry. The study was supported in part by International Research Support Initiative Program (IRSIP) and National research program for universities (NRPU, Project ID: 2989), Higher Education Commission, Pakistan as well as Centre for Translational Cancer Research, Institute of Biosciences, Texas A&M Health Science Centre, Houston, Texas, USA. Publisher Copyright: {\textcopyright} 2019, The Author(s).",
year = "2019",
month = dec,
day = "1",
doi = "10.1038/s41598-019-43093-x",
language = "English (US)",
volume = "9",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "The Author(s) SN -",
number = "1",
}