Influence of dietary trans fatty acids in swine lipoprotein composition and structure

R. L. Jackson, J. D. Morrisett, Henry J. Pownall, Antonio Gotto, A. Kamio, H. Imai, R. Tracy, F. A. Kummerow

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

4 groups of 20 weanling swine each were fed either basal diet, basal plus hydrogenated fat (13% trans), basal plus hydrogenated fat (13% trans) and 0.4% cholesterol, or basal plus beef tallow (all cis). After 6 mth of feeding, the animals were killed and the blood and aortas were removed. Very low density, low density, and high density lipoproteins were then isolated from the plasma by ultracentrifugal flotation. Although the fatty acid composition of the basal diet was different from the diets supplemented with either hydrogenated fat containing trans fatty acid or beef tallow containing all cis, the lipid and fatty acid compositions of each of the isolated lipoprotein classes for the 4 groups of animals were remarkably similar. Elaidate was clearly incorporated into the lipoproteins of animals fed hydrogenated fat, but the level of incorporation was generally less than 5%. In a direct comparison of the structure of the lipoproteins from the different groups, we did not find any significant differences in their physical properties as determined by pyrene fluorescence and electron paramagnetic resonance methods. Grossly visible fatty streaks and fibrous plaques were not found in any of the swine aorta. However, light and electron microscopy indicated the presence of atherosclerotic lesions in the distal abdominal aorta and bifurcation. These studies demonstrate that a diet containing a substantial amount of trans fatty acid leads to a small but definite incorporation into the swine lipoproteins. However, such changes had relatively little effect on lipoprotein structure or the presence of atherosclerotic lesions in these 6 mth old swine.

Original languageEnglish (US)
Pages (from-to)182-190
Number of pages9
JournalJournal of lipid research
Volume18
Issue number2
StatePublished - 1977

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology

Fingerprint Dive into the research topics of 'Influence of dietary trans fatty acids in swine lipoprotein composition and structure'. Together they form a unique fingerprint.

Cite this