Influence of a modified preservation solution in kidney transplantation: A comparative experimental study in a porcine model

Mohammad Golriz, Hamidreza Fonouni, Gani Kuttymuratov, Majid Esmaeilzadeh, Morva Tahmasbi Rad, Parvin Jarahian, Thomas Longerich, Alireza Faridar, Sepehr Abbasi, Arianeb Mehrabi, Marta M. Gebhard

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Background/Objective Currently, due to lack of optimal donors, more marginal organs are transplanted. Therefore, there is a high interest to ameliorate preischemic organ preservation, especially for critical donor organs. In this regard, a new histidine-tryptophane ketoglutarate (HTK-N) solution has been designed and its protective efficacy was compared with the standard preservation solutions—University of Wisconsin solution and standard HTK or Custodiol (Bretschneider's solution). Methods Seventy-two landrace pigs were included into the study, as donors and recipients. The donor kidneys were perfused during explantation with cold University of Wisconsin solution (n = 12), standard HTK (n = 12), or HTK-N solutions (n = 12), kept in the respective preservation solution at 4°C for 30 hours, implanted in the recipient pigs, and reperfused. The pigs survived in daily control for 7 days. The serum creatinine and blood urea nitrogen were assessed in pre- and postreperfusion phase on the 3rd day and 7th day posttransplantation. Additionally, tissue samples were taken to analyze the histopathological degree of tubular injury and regeneration before and after reperfusion. Results The three preservation groups were comparable in age, body weight, and hemodynamic parameters. According to statistical proof, they differed in none of the control parameters. Conclusion Although the new preservation HTK solution is in several points a well-thought-out modification of the standard HTK solution, its preservation efficacy, at least for kidney preservation in a pig model for 30 hours, seems to be comparable to the current used solutions. A real advantage, however, could be confirmed in clinical settings, where marginal organs may influence the clinical outcome.

Original languageEnglish (US)
Pages (from-to)106-115
Number of pages10
JournalAsian Journal of Surgery
Volume40
Issue number2
DOIs
StatePublished - Apr 1 2017

Keywords

  • ischemia reperfusion injury
  • kidney transplantation
  • preservation solution

ASJC Scopus subject areas

  • Surgery

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