TY - JOUR
T1 - Induction of therapeutic gene silencing in leukocyte-implicated diseases by targeted and stabilized nanoparticles
T2 - A mini-review
AU - Peer, Dan
PY - 2010/11/20
Y1 - 2010/11/20
N2 - RNA interference (RNAi) is a highly conserved endogenous mechanism that uses small RNA species to guide the sequence-specific silencing of gene expression. The discovery that RNAi functions in mammalian cells to regulate important cellular processes suggested that harnessing these endogenous gene-silencing pathways can prove to be an effective method for the targeted silencing of gene expression. Yet, the key challenge in translating the discovery of RNAi into a novel therapeutic modality is the lack of effective and safe delivery strategies. Here, we describe the major systemic delivery platforms that have been developed. Focus is given to the development of new strategies to target leukocytes, which are among the most difficult cells to transduce with RNAi. Finally, we discuss our strategies to target subsets of leukocytes using integrin-targeted and stabilized nanoparticles (I-tsNPs).
AB - RNA interference (RNAi) is a highly conserved endogenous mechanism that uses small RNA species to guide the sequence-specific silencing of gene expression. The discovery that RNAi functions in mammalian cells to regulate important cellular processes suggested that harnessing these endogenous gene-silencing pathways can prove to be an effective method for the targeted silencing of gene expression. Yet, the key challenge in translating the discovery of RNAi into a novel therapeutic modality is the lack of effective and safe delivery strategies. Here, we describe the major systemic delivery platforms that have been developed. Focus is given to the development of new strategies to target leukocytes, which are among the most difficult cells to transduce with RNAi. Finally, we discuss our strategies to target subsets of leukocytes using integrin-targeted and stabilized nanoparticles (I-tsNPs).
KW - Integrins
KW - Leukocytes
KW - Nanoparticles
KW - RNAi
KW - SiRNA
UR - http://www.scopus.com/inward/record.url?scp=78649529391&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78649529391&partnerID=8YFLogxK
U2 - 10.1016/j.jconrel.2010.06.029
DO - 10.1016/j.jconrel.2010.06.029
M3 - Article
C2 - 20624432
AN - SCOPUS:78649529391
VL - 148
SP - 63
EP - 68
JO - Journal of Controlled Release
JF - Journal of Controlled Release
SN - 0168-3659
IS - 1
ER -