Induction of immunoglobulin and antigen-dependent antibody synthesis in human lymphocytes, using supernatants from mitogen-stimulated cultures

M. E. North, Malcolm Brenner

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Supernatants derived from pokeweed mitogen-stimulated peripheral blood lymphocytes were freed of lectin by passage over an antibody column: the eluate contained < 2 ng/ml of PWM, measured by radioimmunoassay. In serum-free medium, these supernatants induce B-cell but not T-cell proliferation. Maximum proliferation is seen only if the B cells are co-cultured with irradiated T cells. The supernatants induce polyclonal immunoglobulin production, and in the presence of a soluble protein antigen (tetanus toxoid) they also induce specific antibody synthesis, in donors immunized up to 18 months previously.

Original languageEnglish (US)
Pages (from-to)157-163
Number of pages7
JournalImmunology
Volume48
Issue number1
StatePublished - Jan 1 1983

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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