Abstract
Supernatants derived from pokeweed mitogen-stimulated peripheral blood lymphocytes were freed of lectin by passage over an antibody column: the eluate contained < 2 ng/ml of PWM, measured by radioimmunoassay. In serum-free medium, these supernatants induce B-cell but not T-cell proliferation. Maximum proliferation is seen only if the B cells are co-cultured with irradiated T cells. The supernatants induce polyclonal immunoglobulin production, and in the presence of a soluble protein antigen (tetanus toxoid) they also induce specific antibody synthesis, in donors immunized up to 18 months previously.
Original language | English (US) |
---|---|
Pages (from-to) | 157-163 |
Number of pages | 7 |
Journal | Immunology |
Volume | 48 |
Issue number | 1 |
State | Published - Jan 1 1983 |
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology