Induction of differentiation rescues HL-60 cells from Rana catesbeiana ribonuclease-induced cell death

Chyou Wei Wei; Chih Chi Andrew Hu; Chih Hang Anthony Tang; Ming Chieh Lee; Jaang Jiun Wang

doi:10.1016/S0014-5793(02)03577-9

Induction of differentiation rescues HL-60 cells from Rana catesbeiana ribonuclease-induced cell death

Chyou Wei Wei, Chih Chi Andrew Hu, Chih Hang Anthony Tang, Ming Chieh Lee, Jaang Jiun Wang

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Rana catesbeiana ribonuclease (RC-RNase) exerted strong anti-tumor activity and its cytotoxicity was shown to correlate with differentiation stages of three different hepatoma cell lines. In this study, we demonstrate different RC-RNase cytotoxicity in undifferentiated HL-60 cells and in those that had been induced to differentiate by retinoic acid or dimethylsulfoxide. RC-RNase showed cytotoxicity in undifferentiated HL-60 cells, but not in HL-60 cells undergoing terminal differentiation. Furthermore, the caspase-9/caspase-3 pathway was activated when RC-RNase induced death in undifferentiated HL-60 cells and induction of differentiation led to a reversal of the caspase activation pathway.

Original language	English (US)
Pages (from-to)	421-426
Number of pages	6
Journal	FEBS Letters
Volume	531
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(02)03577-9
State	Published - Nov 20 2002

Keywords

Caspase
Differentiation
HL-60 cell
Rana catesbeiana ribonuclease

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(02)03577-9

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title = "Induction of differentiation rescues HL-60 cells from Rana catesbeiana ribonuclease-induced cell death",

abstract = "Rana catesbeiana ribonuclease (RC-RNase) exerted strong anti-tumor activity and its cytotoxicity was shown to correlate with differentiation stages of three different hepatoma cell lines. In this study, we demonstrate different RC-RNase cytotoxicity in undifferentiated HL-60 cells and in those that had been induced to differentiate by retinoic acid or dimethylsulfoxide. RC-RNase showed cytotoxicity in undifferentiated HL-60 cells, but not in HL-60 cells undergoing terminal differentiation. Furthermore, the caspase-9/caspase-3 pathway was activated when RC-RNase induced death in undifferentiated HL-60 cells and induction of differentiation led to a reversal of the caspase activation pathway.",

keywords = "Caspase, Differentiation, HL-60 cell, Rana catesbeiana ribonuclease",

author = "Wei, {Chyou Wei} and Hu, {Chih Chi Andrew} and Tang, {Chih Hang Anthony} and Lee, {Ming Chieh} and Wang, {Jaang Jiun}",

note = "Funding Information: This research was sponsored by a grant from the National Science Council, R.O.C. (NSC 89-2320-B-016-109). ",

year = "2002",

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doi = "10.1016/S0014-5793(02)03577-9",

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T1 - Induction of differentiation rescues HL-60 cells from Rana catesbeiana ribonuclease-induced cell death

AU - Wei, Chyou Wei

AU - Hu, Chih Chi Andrew

AU - Tang, Chih Hang Anthony

AU - Lee, Ming Chieh

AU - Wang, Jaang Jiun

N1 - Funding Information: This research was sponsored by a grant from the National Science Council, R.O.C. (NSC 89-2320-B-016-109).

PY - 2002/11/20

Y1 - 2002/11/20

N2 - Rana catesbeiana ribonuclease (RC-RNase) exerted strong anti-tumor activity and its cytotoxicity was shown to correlate with differentiation stages of three different hepatoma cell lines. In this study, we demonstrate different RC-RNase cytotoxicity in undifferentiated HL-60 cells and in those that had been induced to differentiate by retinoic acid or dimethylsulfoxide. RC-RNase showed cytotoxicity in undifferentiated HL-60 cells, but not in HL-60 cells undergoing terminal differentiation. Furthermore, the caspase-9/caspase-3 pathway was activated when RC-RNase induced death in undifferentiated HL-60 cells and induction of differentiation led to a reversal of the caspase activation pathway.

AB - Rana catesbeiana ribonuclease (RC-RNase) exerted strong anti-tumor activity and its cytotoxicity was shown to correlate with differentiation stages of three different hepatoma cell lines. In this study, we demonstrate different RC-RNase cytotoxicity in undifferentiated HL-60 cells and in those that had been induced to differentiate by retinoic acid or dimethylsulfoxide. RC-RNase showed cytotoxicity in undifferentiated HL-60 cells, but not in HL-60 cells undergoing terminal differentiation. Furthermore, the caspase-9/caspase-3 pathway was activated when RC-RNase induced death in undifferentiated HL-60 cells and induction of differentiation led to a reversal of the caspase activation pathway.

KW - Caspase

KW - Differentiation

KW - HL-60 cell

KW - Rana catesbeiana ribonuclease

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ER -

Induction of differentiation rescues HL-60 cells from Rana catesbeiana ribonuclease-induced cell death

Abstract

Keywords

ASJC Scopus subject areas

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