Induction of differentiation rescues HL-60 cells from Rana catesbeiana ribonuclease-induced cell death

Chyou Wei Wei, Chih Chi Andrew Hu, Chih Hang Anthony Tang, Ming Chieh Lee, Jaang Jiun Wang

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Rana catesbeiana ribonuclease (RC-RNase) exerted strong anti-tumor activity and its cytotoxicity was shown to correlate with differentiation stages of three different hepatoma cell lines. In this study, we demonstrate different RC-RNase cytotoxicity in undifferentiated HL-60 cells and in those that had been induced to differentiate by retinoic acid or dimethylsulfoxide. RC-RNase showed cytotoxicity in undifferentiated HL-60 cells, but not in HL-60 cells undergoing terminal differentiation. Furthermore, the caspase-9/caspase-3 pathway was activated when RC-RNase induced death in undifferentiated HL-60 cells and induction of differentiation led to a reversal of the caspase activation pathway.

Original languageEnglish (US)
Pages (from-to)421-426
Number of pages6
JournalFEBS Letters
Volume531
Issue number3
DOIs
StatePublished - Nov 20 2002

Keywords

  • Caspase
  • Differentiation
  • HL-60 cell
  • Rana catesbeiana ribonuclease

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Fingerprint Dive into the research topics of 'Induction of differentiation rescues HL-60 cells from Rana catesbeiana ribonuclease-induced cell death'. Together they form a unique fingerprint.

Cite this