Induction of cytochrome P-450 related metabolic activities in the rat ventral prostate

Peter Söderkvist, Rune Toftgård, Jan Åke Gustafsson

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Rats were treated with β-naphthoflavone (BNF) or phenobarbital (PB), 80 mg kg i.p. for 4 days and microsomes prepared from the ventral prostate were assayed for aryl hydrocarbon hydroxylase (AHH), 7-ethoxyresorufin-O-deethylase (7-EOD) and NADPH-cytochrome c reductase activities. The relative increase after BNF treatment was approx. 1000 times for AHH, and 800 times for 7-EOD, while the NADPH-cytochrome c reductase activity was not significantly altered. PB treatment caused no significant effects. Treatment with BNF led to an increased in vitro formation of all measured benzo(a)pyrene (B(a)P) metabolites, especially phenols. Carbon monoxide (CO) and α-naphthoflavone (α-NF) inhibited 7-EOD- and AHH-activities. The rat ventral prostate contains inducible cytochrome P-450-dependent enzymes, a circumstance of potential importance in the etiology of prostatic carcinoma.

Original languageEnglish (US)
Pages (from-to)61-69
Number of pages9
JournalToxicology Letters
Volume10
Issue number1
DOIs
StatePublished - Jan 1982

ASJC Scopus subject areas

  • Toxicology

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