Induction of colitis-associated neoplasia in mice using azoxymethane and dextran sodium sulfate

David C. Montrose, Tomoki Makino, Srijani Basu, Naotake Ito, Andrew J. Dannenberg

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Long-standing inflammatory bowel diseases (IBD) increase the risk for the development of colorectal cancer (CRC). This increase is due in large part to chronic intestinal inflammation which exposes the epithelium to pro-carcinogenic factors. Moreover, enhanced mucosal proliferation associated with repetitive wound healing events following an inflammatory episode, further enhance this pro-tumorigenic environment. Although multiple factors involved in IBD pathogenesis and its associated neoplasia have been identified, more work is needed to develop and improve therapies to ameliorate disease and thus reduce CRC risk. Murine models have served as useful tools to identify factors involved in the pathogenesis of colitis-associated neoplasia and test therapies. These include both chemically-induced and genetic engineering approaches, resulting in chronic inflammation and tumor development. Here, we present a step-by-step method of inducing inflammation-associated colon neoplasia by combining administration of azoxymethane and dextran sodium sulfate in mice. A detailed description of this methodology will facilitate its use in the scientific community with the goals of further elucidating the mechanisms underlying colitis-associated tumorigenesis and developing risk reducing interventions.

Original languageEnglish (US)
Title of host publicationCarcinogen-driven mouse models of oncogenesis
EditorsLorenzo Galluzzi, Lorenzo Galluzzi, Lorenzo Galluzzi, Aitziber Buqué
PublisherAcademic Press
Pages123-135
Number of pages13
ISBN (Print)9780128225349
DOIs
StatePublished - Jan 2021

Publication series

NameMethods in Cell Biology
Volume163
ISSN (Print)0091-679X

Keywords

  • Colorectal cancer
  • Gastrointestinal
  • Inflammation
  • Mouse model
  • Tumor

ASJC Scopus subject areas

  • Cell Biology

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