Induction of both cytochromes P-450 and P-448 by 2,3′,4,4′,5-pentabromobiphenyl, a component of fireMaster

L. W. Robertson, A. Parkinson, S. Safe

Research output: Contribution to journalArticle

46 Scopus citations

Abstract

The synthesis and purification of a component of fireMaster BP-6 and fireMaster FF-1, 2,3′,4,4′,5-pentabromobiphenyl, is described. The compound was found to be a potent inducer of liver microsomal drug-metabolizing enzymes in the rat, enhancing those enzymic activities induced by both phenobarbitone and 3-methylcholanthrene (i.e. cytochromes P-450 and P-448). The pentabromobiphenyl enhanced the activities of benzo[a]pyrene hydroxylase, dimethylamino-antipyrine N-demethylase and NADPH-cytochrome c reductase. The hepatic cytochromes b5 and P-450 were increased and the Soret peak maximum of the latter was shifted to 448.5 nm. The relative peak intensities and spectral shifts for the ethylisocyanide-binding difference spectra confirmed the mixed induction characteristics of 2,3′,4,4′,5-pentabromobiphenyl.

Original languageEnglish (US)
Pages (from-to)175-182
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume92
Issue number1
DOIs
StatePublished - Jan 15 1980

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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