Indirect determination of protein content by inductively coupled plasma-mass spectrometry

Meng Wang, Wei Yue Feng, Wen Wei Lu, Bai Li, Bing Wang, Mo Tao Zhu, Yun Wang, Yu Liang Zhao, Zhi Fang Chai

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Three standard proteins including bovine serum albumin (BSA), superoxide dismutase (SOD) and metallothionein (MT) were separated by size exclusion chromatography and on-line determined via sulfur by inductively coupled plasma-mass spectrometry. Molar ratios of the proteins were calculated by the analytical results and the known sulfur content in proteins. The mobile phase was 0. 1 mol/L Tris-HAc. In order to avoid the polyatomic interferences on the sulfur determination, oxygen was added into the collision cell and reacted with sulfur to form sulfur-oxide ion. Therefore, the intensity of 32S 16O + represented sulfur content during the measurement. These results were in good agreement with the gravimetric results. The RSDs for the proteins are less than 3% and the detection limits for BSA, SOD and MT-II are 14, 52 and 27 pmol, respectively.

Original languageEnglish (US)
Pages (from-to)321-324
Number of pages4
JournalFenxi Huaxue/ Chinese Journal of Analytical Chemistry
Volume36
Issue number3
StatePublished - Mar 2008

Keywords

  • Collision cell
  • Inductively coupled plasma-mass spectrometry
  • Protein quantification
  • Size exclusion chromatography
  • Sulfur

ASJC Scopus subject areas

  • Analytical Chemistry

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