Independent elicitation of the oxidative burst and phytoalexin formation in cultured plant cells

David Davis; Jeffery Merida; Laurent Legendre; Philip Low; Peter Heinstein

doi:10.1016/S0031-9422(00)95144-6

Independent elicitation of the oxidative burst and phytoalexin formation in cultured plant cells

David Davis, Jeffery Merida, Laurent Legendre, Philip Low, Peter Heinstein

Research output: Contribution to journal › Article › peer-review

66 Scopus citations

Abstract

Analysis of components in a crude elicitor preparation (AP-elicitor) from the phytopathogenic fungus Verticillium dahliae 277 demonstrated that a protein moiety appears to be the specific extracellular signal responsible for phytoalexin induction in cotton and soybean cell suspension cultures. However, the carbohydrate portion of the AP-elicitor appeared to be responsible for the induction of the hydrogen peroxide burst. A similar analysis of the endogeneous elicitor polygalacturonic acid (degree of polymerization = 20) showed that only the oxidative activity was induced in soybean and cotton cells but not the formation of phytoalexins.

Original language	English (US)
Pages (from-to)	607-611
Number of pages	5
Journal	Phytochemistry
Volume	32
Issue number	3
DOIs	https://doi.org/10.1016/S0031-9422(00)95144-6
State	Published - Feb 1993

Keywords

cell suspension cultures
elicitation
Glycine max
Gossypium hirsutum
Leguminosae
Malvaceae
oxidative burst.
phytoalexins
Verticillium dahliae

ASJC Scopus subject areas

Drug Discovery
Organic Chemistry
Molecular Biology
Biochemistry
Plant Science

Access to Document

10.1016/S0031-9422(00)95144-6

Cite this

@article{7aba44941f47490caffdedea5c82fda4,

title = "Independent elicitation of the oxidative burst and phytoalexin formation in cultured plant cells",

abstract = "Analysis of components in a crude elicitor preparation (AP-elicitor) from the phytopathogenic fungus Verticillium dahliae 277 demonstrated that a protein moiety appears to be the specific extracellular signal responsible for phytoalexin induction in cotton and soybean cell suspension cultures. However, the carbohydrate portion of the AP-elicitor appeared to be responsible for the induction of the hydrogen peroxide burst. A similar analysis of the endogeneous elicitor polygalacturonic acid (degree of polymerization = 20) showed that only the oxidative activity was induced in soybean and cotton cells but not the formation of phytoalexins.",

keywords = "cell suspension cultures, elicitation, Glycine max, Gossypium hirsutum, Leguminosae, Malvaceae, oxidative burst., phytoalexins, Verticillium dahliae",

author = "David Davis and Jeffery Merida and Laurent Legendre and Philip Low and Peter Heinstein",

year = "1993",

month = feb,

doi = "10.1016/S0031-9422(00)95144-6",

language = "English (US)",

volume = "32",

pages = "607--611",

journal = "Phytochemistry",

issn = "0031-9422",

publisher = "Elsevier Limited",

number = "3",

}

TY - JOUR

T1 - Independent elicitation of the oxidative burst and phytoalexin formation in cultured plant cells

AU - Davis, David

AU - Merida, Jeffery

AU - Legendre, Laurent

AU - Low, Philip

AU - Heinstein, Peter

PY - 1993/2

Y1 - 1993/2

N2 - Analysis of components in a crude elicitor preparation (AP-elicitor) from the phytopathogenic fungus Verticillium dahliae 277 demonstrated that a protein moiety appears to be the specific extracellular signal responsible for phytoalexin induction in cotton and soybean cell suspension cultures. However, the carbohydrate portion of the AP-elicitor appeared to be responsible for the induction of the hydrogen peroxide burst. A similar analysis of the endogeneous elicitor polygalacturonic acid (degree of polymerization = 20) showed that only the oxidative activity was induced in soybean and cotton cells but not the formation of phytoalexins.

AB - Analysis of components in a crude elicitor preparation (AP-elicitor) from the phytopathogenic fungus Verticillium dahliae 277 demonstrated that a protein moiety appears to be the specific extracellular signal responsible for phytoalexin induction in cotton and soybean cell suspension cultures. However, the carbohydrate portion of the AP-elicitor appeared to be responsible for the induction of the hydrogen peroxide burst. A similar analysis of the endogeneous elicitor polygalacturonic acid (degree of polymerization = 20) showed that only the oxidative activity was induced in soybean and cotton cells but not the formation of phytoalexins.

KW - cell suspension cultures

KW - elicitation

KW - Glycine max

KW - Gossypium hirsutum

KW - Leguminosae

KW - Malvaceae

KW - oxidative burst.

KW - phytoalexins

KW - Verticillium dahliae

UR - http://www.scopus.com/inward/record.url?scp=0000131019&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0000131019&partnerID=8YFLogxK

U2 - 10.1016/S0031-9422(00)95144-6

DO - 10.1016/S0031-9422(00)95144-6

M3 - Article

AN - SCOPUS:0000131019

VL - 32

SP - 607

EP - 611

JO - Phytochemistry

JF - Phytochemistry

SN - 0031-9422

IS - 3

ER -

Independent elicitation of the oxidative burst and phytoalexin formation in cultured plant cells

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this