Povyshenie chuvstvitel'nosti immunogistokhimicheskogo metoda vyiavleniia adduktov kantserogenov s DNK.

Translated title of the contribution: Increasing the sensitivity of an immunohistochemical method for detecting carcinogen adducts with DNA

Andrei M Mikheev, M. Asamoto

Research output: Contribution to journalArticle

Abstract

Application of polyclonal antibodies against N7-methylguanine (N7-MG) for immunohistochemical detection of the DNA damage following exposure to methylnitrosourea in cultured cells (CHO and IAR-27) has been shown. The approaches were applied: a) an extraction of nuclei, and b) a silver intensification of the end product of peroxidase reaction. Both the approaches were effective in increasing the sensitivity of immunohistochemical detection of N7-MG, the latter technique being most sensitive. It is proposed that the silver intensification technique is very perspective to have a high levels of sensitivity and specificity, a sufficient contrast and preservation of the cell morphology. The efficiency of the new approach in application to the purposes of molecular epidemiology is discussed.

Translated title of the contributionIncreasing the sensitivity of an immunohistochemical method for detecting carcinogen adducts with DNA
Original languageRussian
Pages (from-to)62-66
Number of pages5
JournalTsitologiya
Volume34
Issue number3
StatePublished - Jan 1 1992

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

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