TY - JOUR
T1 - Increased cortical bone mineral content but unchanged trabecular bone mineral density in female mice
AU - Windahl, S. H.
AU - Vidal, O.
AU - Andersson, G.
AU - Gustafsson, J. A.
AU - Ohlsson, C.
N1 - Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 1999/10
Y1 - 1999/10
N2 - Ovariectomy in young, growing rodents results in decreased trabecular bone mineral density (BMD) and increased radial growth of the cortical bone. Both of these effects are reversed by treatment with estrogen. The aim of the present study was to determine the physiological role of estrogen receptorβ (ERβ) on bone structure and bone mineral content (BMC). The BMC was increased in adult (11 weeks old), but not prepubertal (4 weeks old), female ERβ(-/-) mice compared with wild-type (WT) mice. This increase in BMC in females was not due to increased trabecular BMD, but to an increased cross- sectional cortical bone area associated with a radial bone growth. Male ERβ(-/-) mice displayed no bone abnormalities compared with WT mice. Ovariectomy decreased the trabecular BMD to the same extent in adult female ERβ(-/-) mice as in WT mice. The expression levels of osteoblast associated genes α1(I) collagen, alkaline phosphatase, and osteocalcin mRNAs - were elevated in bone from adult ERβ(-/-) females compared with WT mice. These observations provide a possible explanation for the increased radial bone growth seen in female mutants, suggesting a repressive function for ERβ in the regulation of bone growth during female adolescence. In summary, ERβ is essential for the pubertal feminization of the cortical bone in female mice but is not required for the protective effect of estrogens on trabecular BMD.
AB - Ovariectomy in young, growing rodents results in decreased trabecular bone mineral density (BMD) and increased radial growth of the cortical bone. Both of these effects are reversed by treatment with estrogen. The aim of the present study was to determine the physiological role of estrogen receptorβ (ERβ) on bone structure and bone mineral content (BMC). The BMC was increased in adult (11 weeks old), but not prepubertal (4 weeks old), female ERβ(-/-) mice compared with wild-type (WT) mice. This increase in BMC in females was not due to increased trabecular BMD, but to an increased cross- sectional cortical bone area associated with a radial bone growth. Male ERβ(-/-) mice displayed no bone abnormalities compared with WT mice. Ovariectomy decreased the trabecular BMD to the same extent in adult female ERβ(-/-) mice as in WT mice. The expression levels of osteoblast associated genes α1(I) collagen, alkaline phosphatase, and osteocalcin mRNAs - were elevated in bone from adult ERβ(-/-) females compared with WT mice. These observations provide a possible explanation for the increased radial bone growth seen in female mutants, suggesting a repressive function for ERβ in the regulation of bone growth during female adolescence. In summary, ERβ is essential for the pubertal feminization of the cortical bone in female mice but is not required for the protective effect of estrogens on trabecular BMD.
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U2 - 10.1172/JCI6730
DO - 10.1172/JCI6730
M3 - Article
C2 - 10510330
AN - SCOPUS:0032748023
SN - 0021-9738
VL - 104
SP - 895
EP - 901
JO - Journal of Clinical Investigation
JF - Journal of Clinical Investigation
IS - 7
ER -