Purpose: Malignant tumours develop strategies to avoid immune recognition and elimination by T cells, even in individuals with a fully functioning immune system. To explore the treatment approach of adoptive immunotherapy, we exploited T cells loaded with radiolabelled gold nanoparticles (AuNPs) to track T cells in vivo. Materials and methods: Surface-modified AuNPs were radiolabelled with 111In or 64Cu. They were then transferred into T cells via electroporation. To evaluate the effectiveness of this process, T cells loaded with 111In-radiolabelled AuNPs were injected directly into the right lung of nude mice for in vivo imaging by micro-SPECT/CT. T cells loaded with 64Cu-radiolabelled AuNPs were then injected into the tail vein of nude mice and imaged by micro-PET/CT. Results: High uptake signals were observed in the right lung following the direct injection of T cells containing 111In-labelled AuNPs. Imaging showed a marked difference in the dynamic biodistribution of T cells containing 64Cu-labelled AuNPs when compared with 64Cu-labelled AuNPs alone. Conclusions: This study demonstrated the feasibility of the in vivo imaging of T cells loaded with radiolabelled AuNPs.
- Gold nanoparticles
- In vivo imaging
- T cells
ASJC Scopus subject areas
- Radiology Nuclear Medicine and imaging