TY - GEN
T1 - In vivo ICAM-1 directed molecular imaging of tumor, inflamed milieu, and acute inflammation
AU - Chen, Xiaoyue
AU - Wong, Richard
AU - Khalidov, Ildar
AU - Wang, Y. Andrew
AU - Wang, Yi
AU - Jin, Moonsoo M.
PY - 2011
Y1 - 2011
N2 - We hypothesized that nanoparticles designed to mimic the molecular interactions occurring between inflamed leukocytes and endothelium may possess specificity toward diverse host inflammatory responses. Here, superparamagnetic iron oxide (SPIO) nanoparticles were conjugated with integrin lymphocyte function-associated antigen (LFA)-1 Inserted (I) domain, engineered to mimic activated LFA-1 in leukocytes. By whole body optical imaging and MRI, we found I domain-coated nanoparticles were localized specifically to the tumors with high ICAM-1 expression as well as to the vasculature with ICAM-1 induction within and in the invasive front of the tumor. Furthermore, with a newly developed MRI technique, we achieved quantitative mapping of nanoparticle distribution in vivo in a mouse model of acute inflammation. This study presents the first demonstration of in vivo detection of tumor-associated vasculature by targeting inflammation with systemically injected nanoparticles, offering a possibility of tumor detection not by tumor surface antigens but by an inflamed milieu present in the tumor microenvironment.
AB - We hypothesized that nanoparticles designed to mimic the molecular interactions occurring between inflamed leukocytes and endothelium may possess specificity toward diverse host inflammatory responses. Here, superparamagnetic iron oxide (SPIO) nanoparticles were conjugated with integrin lymphocyte function-associated antigen (LFA)-1 Inserted (I) domain, engineered to mimic activated LFA-1 in leukocytes. By whole body optical imaging and MRI, we found I domain-coated nanoparticles were localized specifically to the tumors with high ICAM-1 expression as well as to the vasculature with ICAM-1 induction within and in the invasive front of the tumor. Furthermore, with a newly developed MRI technique, we achieved quantitative mapping of nanoparticle distribution in vivo in a mouse model of acute inflammation. This study presents the first demonstration of in vivo detection of tumor-associated vasculature by targeting inflammation with systemically injected nanoparticles, offering a possibility of tumor detection not by tumor surface antigens but by an inflamed milieu present in the tumor microenvironment.
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U2 - 10.1109/NEBC.2011.5778691
DO - 10.1109/NEBC.2011.5778691
M3 - Conference contribution
AN - SCOPUS:79958736353
SN - 9781612848273
T3 - 2011 IEEE 37th Annual Northeast Bioengineering Conference, NEBEC 2011
BT - 2011 IEEE 37th Annual Northeast Bioengineering Conference, NEBEC 2011
T2 - 37th Annual Northeast Bioengineering Conference, NEBEC 2011
Y2 - 1 April 2011 through 3 April 2011
ER -